An immune responsive multidomain galectin from bay scallop Argopectens irradians

Fish & Shellfish Immunology
Xiaoyan SongLinsheng Song

Abstract

Galectins are a family of beta-galactoside-binding lectins which play crucial roles in innate immunity of vertebrates and invertebrates. In the present study, the cDNA of a galectin with multiple carbohydrate-recognition domains (CRDs) was cloned from bay scallop Argopectens irradians (designated AiGal1) by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) techniques. The full-length cDNA of AiGal1 was of 2235 nucleotides, encoding a polypeptide of 549 amino acids. SMART program analysis revealed that AiGal1 contained four galectin CRDs, and all the CRDs contained the two consensus motifs essential for ligand-binding. Quantitative real-time PCR was employed to investigate the tissue distribution of AiGal1 mRNA and temporal expression in haemocytes of scallops challenged with Vibrio anguillarum, Micrococcus luteus and Pichia pastoris. The AiGal1 mRNA could be detected in all tested tissues with the highest expression level in hepatopancreas. After challenged by V. anguillarum and M. luteus, the expression level of AiGal1 mRNA was both up-regulated and reached the maximum level at 9 h (1.52 fold, P < 0.05) and 18 h (2.89 fold, P < 0.01) post challenge, respectively. However, there was no significant differe...Continue Reading

Citations

Aug 7, 2012·Developmental and Comparative Immunology·Lingling WangLinsheng Song
Feb 1, 2012·Fish & Shellfish Immunology·Jae-Sung RheeJae-Seong Lee
Jan 18, 2011·Developmental and Comparative Immunology·Xiaoyan SongXiaolin Liu
Sep 4, 2010·Developmental and Comparative Immunology·Dianchang ZhangTianfeng Su
Dec 3, 2014·Developmental and Comparative Immunology·Alexander M Gorbushin, Elena A Borisova
May 25, 2015·Journal of Invertebrate Pathology·Bassem Allam, David Raftos
Dec 9, 2014·Fish & Shellfish Immunology·Irina V ChikalovetsPavel A Lukyanov
Mar 24, 2015·Fish & Shellfish Immunology·Linsheng SongMengqiang Wang
Feb 24, 2015·Fish & Shellfish Immunology·Marco Gerdol, Paola Venier
Jun 22, 2014·Fish & Shellfish Immunology·Waleska Maldonado-AguayoCristian Gallardo-Escárate
Mar 8, 2018·International Journal of Molecular Sciences·Weilin WangLinsheng Song
Nov 15, 2019·Fish & Shellfish Immunology·Zhouyi ZhangChunlin Wang

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