Jun 1, 1989

An immunochemical marker of low density lipoprotein oxidation

Journal of Lipid Research
Z ZawadzkiY L Marcel

Abstract

Using monoclonal antibodies against apolipoprotein B (apoB) we studied changes in apoB immunoreactivity during copper ion-mediated oxidation of human low density lipoprotein (LDL). The radioimmunoassay experiments demonstrated the decrease of immunoreactivity of three different epitopes of apoB located in different parts of the protein; at the same time the immunoreactivity of another epitope, previously mapped to the C-terminal 20 amino acids of apoB increased markedly during the first 6 h of LDL oxidation and diminished gradually upon prolonged incubation with copper ions. The fate of LDL during oxidation was also monitored using electrophoretic techniques combined with immunodetection. These experiments showed a rapid fragmentation and disappearance of immunoreactive apoB. They also indicated that the diminishing LDL immunoreactivity detectable during oxidation is associated with apoB fragments still attached to the lipid core. The changes in apoB immunoreactivity during Cu2+ treatment of LDL are similar to those observed upon LDL aging. Therefore, it appears that the enhancement of immunoreactivity of the C-terminus of apoB is a general phenomenon associated with various kinds of oxidative modifications of LDL.

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Mentioned in this Paper

Monoclonal Antibodies
Monoclonal antibodies, antineoplastic
Immunoreactivity
Low Density Lipoprotein Cholesterol Measurement
Carboxy-Terminal Amino Acid
Abnormal Fragmented Structure
SDS-PAGE
Amino Acids, I.V. solution additive
Antigenic Specificity
Apolipoproteins B

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