An immunocytochemical investigation of the relationship between substance P and the neurokinin-1 receptor in the lateral horn of the rat thoracic spinal cord

Brain Research
R PollockD J Maxwell

Abstract

The relationship between substance P-containing axons and sympathetic preganglionic neurons possessing the neurokinin-1 receptor was investigated in the lateral horn of the rat thoracic spinal cord. Sympathetic preganglionic neurons were labelled retrogradely with Fluorogold. Sections containing labelled cells were reacted with antibodies against choline acetyltransferase, substance P and the neurokinin-1 receptor and examined with three-colour confocal laser scanning microscopy. In all, 95 sympathetic preganglionic neurons were examined and 79% of these were immunoreactive for the neurokinin-1 receptor. Substance P-immunoreactive axons not only made contacts with preganglionic neurons which were immunoreactive for the receptor but also made contacts with cells which did not express the receptor. Dendrites, labelled with immunoreactivity for choline actyltransferase, also received contacts from substance P-immunoreactive varicosities but this was not related to the presence or the absence of receptor. An electron microscopic analysis was performed to investigate the relationship between substance P-containing boutons and dendrites possessing the neurokinin-1 receptor. Immunoreactivity for substance P was detected with peroxidas...Continue Reading

References

Jun 1, 1992·The Journal of Comparative Neurology·P PilowskyJ Chalmers
Jun 1, 1985·The Journal of Comparative Neurology·B J OldfieldG Nilaver
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S De Biasi, A Rustioni
Oct 31, 1983·Neuroscience Letters·M P GilbeyL P Schramm
Aug 1, 1994·Journal of Neuroscience Methods·C R Anderson, S L Edwards
Mar 22, 1996·Annals of the New York Academy of Sciences·C J Helke, L Yang
Feb 5, 1996·Journal of the Autonomic Nervous System·C Cammack, S D Logan

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Citations

Feb 13, 1999·Progress in Neurobiology·M J Millan
Dec 1, 1998·Neuroscience Letters·C J McNairD J Maxwell
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·A L McLeodA Ribeiro-da-Silva
Dec 28, 2000·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·S OhtoriH Moriya

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