An immunocytochemical study for lysosomal cathepsins B and D related to the intracellular degradation of titanium at the bone-titanium interface

Journal of Periodontology
Y AyukawaT Tanaka

Abstract

The morphological relationship between titanium and lysosomal proteinases, cathepsins B and D, at the bone-titanium interface using titanium-coated plastic implants placed for 28 days in the tibiae of 6-week-old rats was immunocytochemically investigated by the colloidal immunogold-silver method. Under light microscopy the titanium layer appeared to make direct contact with the bone and one or a few layers of slender cells were interposed between the bone and titanium. Ultrastructurally, the titanium came in contact with the bone or the slender cell layer through a 20 to 40 nm thin amorphous zone. The slender cells at the bone-titanium interface consisted of two types; one was an osteoblast type with glycogen granules which was found along the newly-formed bone facing titanium layer. The other was a fibroblast type which came in contact with the titanium layer and occasionally endocytosed the detached titanium fragments. In addition, some of the slender cells also showed degenerative changes. Immunocytochemically, cathepsins B and/or D were sometimes colocalized in some phagolysosomes with titanium fragments. These findings suggested that the fibroblast types at the bone-titanium interface may act as scavengers to remove both c...Continue Reading

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Citations

Aug 10, 2006·Journal of Materials Science. Materials in Medicine·H KawaharaS Matsuda
Jan 23, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·E OsanoY Takahashi
Aug 12, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Stefan RammeltAchim Biewener
Jun 21, 2005·Journal of Materials Science. Materials in Medicine·A C QueirozF J Monteiro
Jul 3, 2019·Journal of Clinical Medicine·André Antonio PelegrineAna Paula Rosifini Alves Claro

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