Dec 12, 2007

An immunohistochemical method for identifying fibroblasts in formalin-fixed, paraffin-embedded tissue

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Tracy GoodpasterHilary A Coller

Abstract

Fibroblasts are critical for tissue homeostasis, and their inappropriate proliferation and activation can result in common and debilitating conditions including fibrosis and cancer. We currently have a poor understanding of the mechanisms that control the growth and activation of fibroblasts in vivo, in part because of a lack of suitable fibroblast markers. We have taken advantage of an antibody previously shown to stain stromal cells in frozen tissues (TE-7) and identified conditions in which it can be used to stain fibroblasts and myofibroblasts in the paraffin-embedded tissue samples routinely collected for pathological analysis. We show that this antibody recognizes growing and quiescent fibroblasts and myofibroblasts by immunohistochemistry, immunofluorescence, and ELISA assays. We also present its staining patterns in normal tissue samples and in breast tumors.

  • References20
  • Citations56

Citations

Mentioned in this Paper

Immunofluorescence Assay
Immunohistochemistry
Specimen Type - Fibroblasts
Formalin
Formol
Fixatives
Immunocytochemistry
Soleus Muscle Structure
Myofibroblasts
Tissue Homeostasis

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