An Immunohistochemical Study of Inflammatory Cell Changes and Matrix Remodeling With and Without Acute Hydrops in Keratoconus

Investigative Ophthalmology & Visual Science
Jennifer C Fan GaskinTrevor Sherwin

Abstract

To determine the inflammatory cell and matrix changes in advanced keratoconus, including acute hydrops, using immunohistochemical analysis. The corneal tissue from eight subjects with keratoconus undergoing corneal transplantation (three keratoconic buttons, five buttons post acute hydrops—one of them with extensive neovascularization following hydrops) was compared with tissue from two normal corneoscleral rims (n = 10). The corneas were sectioned and analyzed with specific markers for macrophages, lymphocytes, dendritic cells, and scar associated matrix molecules laminin, fibronectin, tenascin-C, and type III collagen. Populations of cells using markers for macrophages, leucocytes and antigen presenting cells were found to be associated with the epithelium and stroma of keratoconic tissue. Populations of these cells appeared decreased in hydrops-associated keratoconus except for a large increase in leucocytes in the stroma and endothelium associated with neovascularization. Extracellular matrix deposition was found to be uniquely demonstrated in localized areas of the stroma, corresponding to the site of hydrops involvement. Immunohistochemical analysis revealed a chronic, inflammatory process with recruitment of immunoinflam...Continue Reading

Citations

Feb 13, 2016·Veterinary Ophthalmology·Samantha L PedersonTammy M Michau
Dec 24, 2015·Journal of Ophthalmology·Jingjing YouMichele C Madigan
Jul 11, 2016·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Hatice Nur ColakBulent Gurler
Jul 17, 2016·American Journal of Ophthalmology·Jay J MeyerCharles N J McGhee
Dec 19, 2017·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Wenjia XieYu-Feng Yao
Apr 30, 2017·The British Journal of Ophthalmology·Natasha Kishore PahujaArkasubhra Ghosh
Mar 3, 2017·BioMed Research International·Mariam Lotfy KhaledYutao Liu
Aug 14, 2020·Ocular Immunology and Inflammation·I-Ping Loh, Trevor Sherwin
Aug 30, 2018·Journal of Ophthalmology·Raffaele NuzziFederico Tridico
Dec 8, 2020·Asia-Pacific Journal of Ophthalmology·Alexandra Z CrawfordSue E Ormonde

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