PMID: 6298636Mar 3, 1983

An immunologically active chimaeric protein containing herpes simplex virus type 1 glycoprotein D

Nature
J H WeisR J Watson

Abstract

Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) cause both persistent and latent infections, including recurrent cutaneous disease, lethal neonatal disease, central nervous system disease and other clinical syndromes. Modified live vaccines or conventionally prepared subunit vaccines have generally been unsuccessful in the treatment of HSV-1 and HSV-2 infections from the standpoints of safety and efficacy. It has been established that HSV-1 and HSV-2 infectivity may be neutralized in vitro with antisera directed specifically against each of the four major glycoproteins of the virus (gA/gB, gC, gD and gE) and antisera against glycoprotein gD, of either HSV-1 or HSV-2, are capable of neutralizing both HSV-1 and HSV-2 infectivity in vitro and in vivo. We have previously reported on the identification, DNA sequence and expression at low level in Escherichia coli of the gD gene of HSV-1 strain Patton. Here we describe construction of a hybrid gene encoding a chimaeric protein containing HSV-1 gD, bacteriophage lambda Cro and E. coli beta-galactosidase (gD-beta-gal) protein, which is expressed at high level in E. coli. Moreover, the chimaeric protein elicits antibodies in rabbits that not only immunoprecipitate gD from cells i...Continue Reading

References

Jan 1, 1986·Archives of Virology·Y KinoR Mori
Jan 1, 1984·Pharmacology & Therapeutics·S A NarangF Georges
May 1, 1991·Bio/technology·D L Wilkinson, R G Harrison
Jan 1, 1985·Antiviral Research·B Meignier, B Roizman
Jan 1, 1988·Journal of the American Academy of Dermatology·F Pass
Aug 15, 1988·FEBS Letters·C H KockenS Welling-Wester

Citations

Nov 1, 1978·Journal of Virology·H S MarsdenN M Wilkie
Jan 1, 1979·Methods in Enzymology·F Bolivar, K Backman
Apr 1, 1980·Analytical Biochemistry·J LangridgeP L Bergquist
Jan 1, 1980·Current Topics in Microbiology and Immunology·B Norrild
Feb 1, 1981·The Journal of Cell Biology·R G AndersonJ L Goldstein
Feb 1, 1982·The Journal of General Virology·R G HopeH S Marsden
Sep 1, 1980·Diabetes·J M Podskalny, C R Kahn

Related Concepts

Viral Vaccines
GLB1
Alkalescens-Dispar Group
Vaccines, Attenuated
Human Herpesvirus 2
Human herpesvirus 1
Glycoproteins
Viral Proteins
Immune Sera
Neonatal Herpes

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