An impediment to random walk: trehalose microenvironment drives preferential endocytic uptake of plasmonic nanoparticles

Chemical Science
Soumik SiddhantaIshan Barman

Abstract

Developing effective theranostic nanoplex platforms for personalized disease treatment necessitates an understanding of and the ability to control live cell-nanoparticle interactions. However, aggregation of nanoparticles on the cell surface and their subsequent internalization is sparsely understood and adversely impact cellular recognition and viability. Here we report a facile method of precisely modulating the aggregation and uptake for silver nanoparticles without altering their surface geometry or functionalization. Exploiting the stabilization properties of trehalose, our approach enables uptake of nanoparticles while reducing aggregation on cell surface and maintaining cell viability. Electron microscopy reveals the larger utilization of endosomal structures in the trehalose-rich environment compared to the nanoparticles' "free" cytosolic diffusion patterns in the control group. Additionally, in the presence of trehalose, plasmon-enhanced Raman spectroscopy confirms the preservation of the protein structure in the vicinity of the nanoparticles reinforcing the promise of the proposed route for label-free, multiplexed intracellular monitoring.

References

Jul 20, 2004·Nature Biotechnology·Xiaohu GaoShuming Nie
Oct 25, 2007·Clinical Pharmacology and Therapeutics·L ZhangO C Farokhzad
Mar 5, 2008·Methods in Molecular Biology·Edward M Perkins, J Michael McCaffery
Feb 12, 2009·ACS Nano·Paola NativoMathias Brust
Apr 23, 2009·Analytical and Bioanalytical Chemistry·Mohammad Kamal HossainYukihiro Ozaki
Jun 11, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Soohee KimDoug-Young Ryu
Oct 13, 2009·Biochemical and Biophysical Research Communications·Ying ChenMartin G Pomper
May 22, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Laura K Braydich-StolleMarie-Claude Hofmann
Jul 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Anil K KodaliRohit Bhargava
Sep 8, 2010·Nanomedicine : Nanotechnology, Biology, and Medicine·Molly K GregasTuan Vo-Dinh
Oct 13, 2010·Nanoscale·Ashwinkumar BhirdeXiaoyuan Chen
Feb 4, 2011·Journal of the American Chemical Society·Marco P MonopoliKenneth A Dawson
Jun 23, 2011·ACS Nano·Alexandre Albanese, Warren C W Chan
Jul 2, 2011·Nanomedicine·Jesse V JokerstSanjiv S Gambhir
Dec 6, 2012·Nature Nanotechnology·Marco P MonopoliKenneth A Dawson
Apr 9, 2014·Angewandte Chemie·Sebastian Schlücker

❮ Previous
Next ❯

Citations

Jul 20, 2017·Chembiochem : a European Journal of Chemical Biology·Jack T BraggMargot G Paulick
Nov 11, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Suman MandalNikhil R Jana

❮ Previous
Next ❯

Methods Mentioned

BETA
Raman spectroscopy
electron microscopy
transmission electron microscopy
fluorescence imaging
dynamic light scattering

Software Mentioned

Zeiss LSM
Origin

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.