An important determinant of the ability of Turnip mosaic virus to infect Brassica spp. and/or Raphanus sativus is in its P3 protein

The Journal of General Virology
Noriko SuehiroSeiichi Okuda

Abstract

Turnip mosaic virus (TuMV, genus Potyvirus, family Potyviridae) infects mainly cruciferous plants. Isolates Tu-3 and Tu-2R1 of TuMV exhibit different infection phenotypes in cabbage (Brassica oleracea L.) and Japanese radish (Raphanus sativus L.). Infectious full-length cDNA clones, pTuC and pTuR1, were constructed from isolates Tu-3 and Tu-2R1, respectively. Progeny virus derived from infections with pTuC induced systemic chlorotic and ringspot symptoms in infected cabbage, but no systemic infection in radish. Virus derived from plants infected with pTuR1 induced a mild chlorotic mottle in cabbage and infected radish systemically to induce mosaic symptoms. By exchanging genome fragments between the two virus isolates, the P3-coding region was shown to be responsible for systemic infection by TuMV and the symptoms it induces in cabbage and radish. Moreover, exchanges of smaller parts of the P3 region resulted in recombinants that induced complex infection phenotypes, especially the combination of pTuC-derived N-terminal sequence and pTuR1-derived C-terminal sequence. Analysis by tissue immunoblotting of the inoculated leaves showed that the distributions of P3-chimeric viruses differed from those of the parents, and that the or...Continue Reading

References

Jan 1, 1992·The Journal of General Virology·J L RiechmannJ A García
Dec 1, 1991·Virology·E Rodríguez-Cerezo, J G Shaw
Jan 1, 1996·Archives of Virology·K OhshimaN Sako
Jul 22, 1998·The Plant Journal : for Cell and Molecular Biology·J C CarringtonM C Schaad
Nov 20, 1998·The Journal of General Virology·M MorenoE Rodríguez-Cerezo
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·S A WhithamJ C Carrington
Jan 7, 2000·Molecular Plant-microbe Interactions : MPMI·M L Rajamäki, J P Valkonen
Apr 8, 2000·Molecular Plant-microbe Interactions : MPMI·J H HämäläinenJ P Valkonen
Feb 28, 2001·Virus Research·S Urcuqui-InchimaF Bernardi
Jun 2, 2001·Molecular Plant-microbe Interactions : MPMI·B Borgstrøm, I E Johansen
Jan 19, 2002·Journal of Virology·Pilar SáenzJuan Antonio García
Mar 6, 2002·Molecular Plant-microbe Interactions : MPMI·Charlotte Kristiane HjulsagerI Elisabeth Johansen
Jul 10, 2003·Molecular Plant-microbe Interactions : MPMI·Frédéric ReversThierry Candresse
Aug 9, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·S L HughesJ A Walsh
Sep 16, 2003·Molecular Plant-microbe Interactions : MPMI·Carol E JennerJohn A Walsh
Oct 24, 2008·Phytopathology·L StavoloneJ F Laliberté
Sep 1, 2002·Molecular Plant Pathology·John A Walsh, Carol E Jenner

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Citations

Nov 2, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yo-Hei KanekoChikara Masuta
Jun 15, 2007·Virus Genes·Sarasate EiamtanasateYun-Kiam Yap
Mar 2, 2005·Journal of Virological Methods·Noriko SuehiroTomohide Natsuaki
Jun 12, 2013·Molecular Plant-microbe Interactions : MPMI·Alberto CarbonellCarmen Simón-Mateo
Sep 16, 2011·Molecular Plant-microbe Interactions : MPMI·Minoru TakeshitaChikara Masuta
Mar 24, 2005·Molecular Plant-microbe Interactions : MPMI·Ida Bagus AndikaTetsuo Tamada
Dec 7, 2007·Molecular Plant-microbe Interactions : MPMI·B SalvadorC Simón-Mateo
Nov 15, 2013·BMC Evolutionary Biology·Julia HillungJosé M Cuevas
Jun 12, 2008·PloS One·Patricia Agudelo-RomeroSantiago F Elena
Dec 4, 2012·Viruses·Jiri SochorRene Kizek
Jan 10, 2014·Molecular Plant-microbe Interactions : MPMI·Laurence Svanella-DumasThierry Candresse
Oct 28, 2015·Molecular Biology and Evolution·Nikon VassilakosBenoît Moury
Dec 10, 2015·Molecular Plant-microbe Interactions : MPMI·Flora SánchezFernando Ponz
Aug 19, 2008·Molecular Plant Pathology·Beatriz SalvadorCarmen Simón-Mateo
Oct 16, 2007·Virus Research·James SusaimuthuRobert R Martin
Jan 22, 2005·The Journal of General Virology·Zhongyang TanKazusato Ohshima

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