An Important Role for CD4+ T Cells in Adaptive Immunity to Toxoplasma gondii in Mice Lacking the Transcription Factor Batf3.

MSphere
Roxane TussiwandL David Sibley

Abstract

Immunity to Toxoplasma gondii at early stages of infection in C57BL/6 mice depends on gamma interferon (IFN-γ) production by NK cells, while at later stages it is primarily mediated by CD8 T cells. We decided to explore the requirement for CD4 T cells during T. gondii infection in Batf3 -/- mice, which lack CD8α+ dendritic cells (DCs) that are necessary for cross-presentation of cell-associated antigens to CD8 T cells. We show that in this immunodeficient background on a BALB/c background, CD4 T cells become important effector cells and are able to protect Batf3 -/- mice from infection with the avirulent strain RHΔku80Δrop5 Independently of the initial NK cell activation, CD4 T cells in wild-type and Batf3 -/- mice were the major source of IFN-γ. Importantly, memory CD4 T cells were sufficient to provide protective immunity following transfer into Batf3 -/- mice and secondary challenge with the virulent RHΔku80 strain. Collectively, these results show that under situations where CD8 cell responses are impaired, CD4 T cells provide an important alternative immune response to T. gondii IMPORTANCE Toxoplasma gondii is a widespread parasite of animals that causes zoonotic infections in humans. Although healthy individuals generally...Continue Reading

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Citations

Mar 27, 2021·Frontiers in Immunology·Maguy HamieHiba El Hajj
Jun 29, 2021·Frontiers in Immunology·Robin A WelshScheherazade Sadegh-Nasseri
Jul 16, 2021·Frontiers in Immunology·Maguy HamieHiba El Hajj

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Methods Mentioned

BETA
GTPases
FACS
lavage
flow cytometry
fluorescence-activated cell sorting

Software Mentioned

FlowJo
Xenogen Living Image
Prism
GraphPad

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