An important role for pentose cycle in the synthesis of citrulline and proline from glutamine in porcine enterocytes

Archives of Biochemistry and Biophysics
G Wu

Abstract

This study was designed to determine a role of pentose cycle in the provision of NADPH for the synthesis of citrulline and proline from glutamine in porcine enterocytes. Enterocytes from 4-day-old pigs were incubated at 37 degrees C for 0 to 30 min in Krebs-Henseleit bicarbonate buffer (pH 7.4) containing 2 mM glutamine and 5 mM glucose in the presence of 0, 0.1, or 0.25 mM dehydroepiandrosterone (DHEA), a potent inhibitor of glucose-6-phosphate dehydrogenase which is the key regulatory enzyme of pentose cycle. The activity of this cycle was estimated with the use of [1-14C]glucose and [6-14C]glucose. In some experiments, the medium included 2 mM ornithine and 2 mM NH4Cl (no glutamine). About 14% of glucose taken up by enterocytes was metabolized via pentose cycle. The flux from glucose into this cycle was decreased by 70 and 86%, respectively, in the presence of 0.1 and 0.25 mM DHEA compared with its absence. DHEA inhibited the synthesis of ornithine, citrulline, arginine, and proline from glutamine in a concentration-dependent manner, but had no effect on the formation of citrulline and arginine from ornithine. However, DHEA decreased the synthesis of proline from ornithine by 79%. DHEA had no effect on cellular ATP concentra...Continue Reading

Citations

Jun 2, 2012·Frontiers in Bioscience (Landmark Edition)·Bi'e TanGuoyao Wu
Jan 18, 2018·The Journal of Biological Chemistry·Aaron M Hosios, Matthew G Vander Heiden
May 13, 2008·Journal of Animal Science·P R RegmiM Oba
Mar 29, 2000·The Journal of Nutrition·P J ReedsF Jahoor
Jul 27, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·E KarnaJ A Pałka
Sep 12, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·P K Davis, G Wu

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