An imprinting element from the mouse H19 locus functions as a silencer in Drosophila

Nature Genetics
F LykoRenato Paro

Abstract

Genomic imprinting as originally described in Sciara is displayed by many organisms. In mammals, X-inactivation and the parent-of-origin-specific silencing of imprinted genes are examples of this phenomenon. A heritable chromatin structural modification may be the critical mechanism in such instances of chromosome condensation and preferential gene inactivation. H19 is an imprinted gene in which the repressed paternal allele is hypermethylated and the compacted chromatin is relatively resistant to digestion by nucleases. In order to uncover underlying conserved epigenetic mechanisms we have introduced a mouse H19 transgene into Drosophila. We show here that a 1.2-kb H19 upstream sequence functions in cis as a parent-of-origin independent silencing element in Drosophila. Strikingly, this cis-acting element is located within an upstream region that is necessary for H19 imprinting in mice. These results suggest involvement of an evolutionary conserved mechanism in both genes silencing in Drosophila and imprinting in mice.

References

May 9, 1991·Nature·M S BartolomeiS M Tilghman
Nov 1, 1988·Molecular and Cellular Biology·H Yoo-WarrenS M Tilghman
Jan 1, 1988·Biotechnology·V Pirrotta
Sep 6, 1982·FEBS Letters·S Urieli-ShovalA Razin
Sep 1, 1993·Genes & Development·M S BartolomeiS M Tilghman
Apr 1, 1995·Nature Genetics·K D TremblayM S Bartolomei
Apr 1, 1994·Current Opinion in Genetics & Development·A Efstratiadis
Nov 25, 1993·Nature·E LiR Jaenisch
Apr 22, 1993·Nature·A C Ferguson-SmithM A Surani
Jun 1, 1996·Current Opinion in Cell Biology·R M John, M A Surani

❮ Previous
Next ❯

Citations

Jul 21, 1998·Molecular Reproduction and Development·S Banerjee, A Smallwood
Sep 25, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·F Lyko, R Paro
Sep 25, 2001·The Journal of Pathology·M Paulsen, A C Ferguson-Smith
Aug 2, 2001·Reviews in Medical Virology·D Pannell, J Ellis
Aug 28, 1999·Molecular Biotechnology·J M Greally
Nov 26, 2008·Development Genes and Evolution·Matthew AnakaVett K Lloyd
Jun 26, 2012·Molecular Genetics and Genomics : MGG·Lori A McEachern, Vett K Lloyd
Jun 5, 1998·Trends in Genetics : TIG·R D NichollsB Horsthemke
Oct 26, 1999·Trends in Genetics : TIG·R Feil, S Khosla
Aug 5, 1998·Current Opinion in Genetics & Development·Y JiangA L Beaudet
Jun 4, 1998·Current Opinion in Genetics & Development·W Reik, J Walter
Jan 14, 2003·Seminars in Cell & Developmental Biology·Jörn Walter, Martina Paulsen
Nov 14, 1997·Nature Genetics·A Olek, J Walter
Apr 24, 2010·Médecine sciences : M/S·Emilie Poisot, Laurent Théodore
Nov 13, 1998·Human Molecular Genetics·A T Hark, S M Tilghman
Feb 28, 2002·Nucleic Acids Research·Robert A DrewellM Azim Surani
Mar 3, 2005·Genes & Development·Sabine SchmittRenato Paro
Feb 17, 2006·Genes & Development·Anne-Marie MartinezGiacomo Cavalli
Aug 25, 2005·Cold Spring Harbor Symposia on Quantitative Biology·S Schoenfelder, R Paro
Feb 24, 2001·Genome Biology·J Peters
Aug 14, 2009·Development·Deborah KwonJudith A Kassis
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·J D BrentonM A Surani
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·F LykoR Paro
May 9, 2012·Genetics Research International·Lori A McEachern
Aug 5, 1998·Current Opinion in Genetics & Development·R M Winter
Apr 10, 2012·Trends in Ecology & Evolution·Robert A DrewellBenjamin P Oldroyd
Dec 18, 1997·American Journal of Human Genetics·R Feil, G Kelsey
May 24, 2011·Developmental Biology·Folami Y IderaabdullahMarisa S Bartolomei
Jun 22, 2000·Medical Hypotheses·V De FonzoV Parisi
Jul 15, 1998·Current Biology : CB·J Goodrich
Aug 18, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·T UedaH Sasaki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.