PMID: 6164878Oct 1, 1980Paper

An improved approach to histofluorescence using the SPG method for tissue monoamines

Journal of Neuroscience Methods
J C De la Torre

Abstract

A modification of the SPG histofluorescence method for the visualization of monoamines in tissues using cryostat sections is described. This modification has let to consistent standardization of the fluorescent intensity from one tissue section to another while also eliminating a cumbersome step in the procedure. The present procedure results in highly rapid and sensitive visualization of dopamine-, noradrenaline- and serotonin-containing neurons and their axonal varicosities. Processing time from fresh tissue to microscopic examination requires less than 15 min.

References

Jan 1, 1979·Journal of Neural Transmission·J McConnellJ Wood
Oct 1, 1979·The Journal of Surgical Research·L E EdstromJ W Surgeon

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Citations

Jan 1, 1985·Cell and Tissue Research·N Kleitman, M A Holzwarth
Jan 1, 1986·Experimental Brain Research·W J FreedH M Geller
Jan 1, 1988·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·S K MahataA Ghosh
Sep 1, 2005·Anatomy and Embryology·Li-Yuan LiuAn Pen
Jul 8, 1982·Brain Research·T A Milner, R Loy
Jun 1, 1985·Journal of the Autonomic Nervous System·D F Peterson, J E Norvell
Nov 1, 1986·Journal of the Autonomic Nervous System·S L Kenny, M A Ariano
Sep 1, 1990·Journal of the Autonomic Nervous System·P G SmithH Reddy
Aug 1, 1991·Journal of the Autonomic Nervous System·C R Anderson, E M McLachlan
Jun 1, 1986·Brain Research·A J LançaD van der Kooy
Oct 1, 1990·Brain Research. Developmental Brain Research·J KoistinahoS I Rapoport

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