An Improved, Chemically Modified RNA Encoding BMP-2 Enhances Osteogenesis In Vitro and In Vivo

Tissue Engineering. Part a
Wen ZhangElizabeth R Balmayor

Abstract

The use of chemically modified RNA (cmRNA) with increased stability using translation initiator of short untranslated regions (TISU) offers the prospect of finally allowing us to unlock the potent osteogenic properties of BMP-2 in a clinically expedient manner. As noted, delivery of recombinant BMP-2 protein has had modest clinical efficacy, whereas gene delivery is effective but very difficult to translate into human clinical use. This study shows the great potential of cmRNA encoding BMP-2 with TISU in a long-bone critical-sized rat model.

References

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Apr 12, 2018·Tissue Engineering. Part a·Zeljka TrepotecCarsten Rudolph

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Citations

Nov 15, 2018·Tissue Engineering. Part a·Elizabeth R Balmayor, Christopher H Evans
Dec 28, 2018·International Journal of Molecular Sciences·Gabriele RussowJohannes Keller
Jun 20, 2019·Frontiers in Bioengineering and Biotechnology·Hasan UludagAysha Ansari
Mar 21, 2019·Stem Cells Translational Medicine·Zohreh Sadat Badieyan, Todd Evans
Jul 11, 2020·Biomedical Materials·Satheesh ElangovanAliasger K Salem
Jan 26, 2021·Molecular Pharmaceutics·Omnia FayedElizabeth R Balmayor
Apr 30, 2021·Materials Today. Bio·L AndréeS C G Leeuwenburgh
Apr 26, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Pinpin WangChantal Pichon
May 9, 2021·Translational Research : the Journal of Laboratory and Clinical Medicine·Rodolfo E De la VegaElizabeth R Balmayor
May 21, 2021·Advanced Drug Delivery Reviews·Noah Z LairdAliasger K Salem
Jun 7, 2021·Biomaterials·Kevin CollonJay R Lieberman
Jun 26, 2021·Frontiers in Molecular Biosciences·Laila A Damiati, Sarah El-Messeiry
Dec 10, 2021·Biotechnology Journal·Dharmaraj Saleth SidharthanNagarajan Selvamurugan

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Methods Mentioned

BETA
transfection
in vitro transcription
electrophoresis
enzyme-linked immunosorbent assay
dynamic light scattering
ELISA
X-ray
light microscopy
transfections

Software Mentioned

CTAn
GraphPad Prism
BZ
GraphPad
NRecon
II Viewer
II Analyzer
GeneScript

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