PMID: 2115551Jul 3, 1990Paper

An improved conjugation method for controlled covalent coupling of synthetic peptides to proteins using glutaraldehyde in a dialysis method

Journal of Immunological Methods
N ZegersE Claassen

Abstract

Controlled and efficient conjugation of synthetic peptides to proteins, for use in immunization or in assay procedures, is a prerequisite for the immunological applications of synthetic peptides. This study describes a new method of conjugating synthetic peptides to proteins in such a way that no homopolymers of synthetic peptides or proteins occur. To achieve this, the protein is first activated with glutaraldehyde and subsequently excess glutaraldehyde is removed. Then coupling of the synthetic peptide to the activated protein occurs while subsequently the surplus reactive glutaraldehyde groups on the protein are blocked with lysine. Excess free peptide and lysine is then removed by dialysis. This improvement not only results in better defined conjugates when compared to classical glutaraldehyde coupling, but also in the consumption of smaller amounts of synthetic peptide during conjugate formation. When used for immunization we obtained similar and sometimes even better responses with the glutaraldehyde based conjugates than with succinimidyl (MBS) conjugates of the same peptides. The performance of the modified conjugates in ELISA procedures, immunization and immunocytochemistry suggests that they are superior to conjugates...Continue Reading

References

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Citations

Oct 6, 2001·Journal of Peptide Science : an Official Publication of the European Peptide Society·L J CruzO Reyes
May 1, 1992·Molecular Immunology·A AvrameasA D Strosberg
Jan 14, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Rosanna CapparelliDomenico Iannelli
Oct 17, 2014·Journal of Immunoassay & Immunochemistry·Mukesh KumarSubrata Sinha
Nov 24, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Toru InoueTakakiyo Saeki
Jul 1, 1995·Bioconjugate Chemistry·W R Gombotz, D K Pettit
Mar 30, 2001·The Journal of Biological Chemistry·S J WilliamsM A McGuckin
Dec 21, 2018·Journal of Materials Science. Materials in Medicine·Fei GeLin Gui
Feb 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marcela TorresArturo Casadevall
Oct 22, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rena J MayMatthew D Scharff
Feb 1, 1992·The American Journal of Physiology·C M FullerD J Benos

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