PMID: 6159591Mar 25, 1980Paper

An improved direct RNA sequence method; its application to Vicia faba 5.8S ribosomal RNA

Nucleic Acids Research
Y TanakaG G Brownlee

Abstract

We have developed a direct read-off sequencing procedure, based on the method of Stanley and Vassilenko using E. coli 5S ribosomal RNA as a model compound. Radioactive bands were transferred from an acrylamide gel fractionation in the first dimension onto a DEAE-cellulose thin layer plate. After in situ enzymatic digestion with RNase T2, mononucleoside 3',5'-diphosphates were separated in the second dimension by electrophoresis at pH 2.3. Using this two-dimensional procedure the entire sequence of 163 residues of the previously unknown Vicia faba (broad bean) 5.8S ribosomal RNA was deduced.

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Citations

Feb 1, 1990·Journal of Molecular Evolution·R A TorresV Hemleben
Nov 1, 1985·Plant Molecular Biology·F TakaiwaM Sugiura
Jan 1, 1982·Progress in Biophysics and Molecular Biology·A Liljas
Jun 15, 1999·Trends in Microbiology·S M Jazwinski
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Sep 11, 1982·Nucleic Acids Research·E DamsR De Wachter
Jan 22, 1982·Nucleic Acids Research·V A Erdmann
Jan 11, 1983·Nucleic Acids Research·V A ErdmannR De Wachter
Jan 1, 1978·Nucleic Acids Research·V A Erdmann
Jun 25, 1980·Nucleic Acids Research·M W Kilpatrick, R T Walker
Jan 10, 1981·Nucleic Acids Research·V A Erdmann
Sep 11, 1981·Nucleic Acids Research·M W Kilpatrick, R T Walker
Oct 10, 1981·Nucleic Acids Research·L DielsR De Wachter
Oct 24, 1981·Nucleic Acids Research·R N Nazar, A G Wildeman
Oct 15, 1985·European Journal of Biochemistry·T KissF Solymosy
Jan 1, 1984·Nucleic Acids Research·V A ErdmannR De Wachter
Mar 13, 2016·Methods : a Companion to Methods in Enzymology·Maryam Zaringhalam, F Nina Papavasiliou
Oct 2, 2015·Current Protocols in Molecular Biology·Thomas M CarlileWendy V Gilbert
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Apr 6, 1990·Biochimica Et Biophysica Acta· Melekhovets YuF, A V Troitsky
Jul 30, 1990·Biochimica Et Biophysica Acta·M Z BarciszewskaJ Barciszewski

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