PMID: 38770May 1, 1979

An improved fluorimetric determination of alpha-mannosidase activity in bovine plasma

Australian Veterinary Journal
A J SinclairJ D Allen

Abstract

This paper describes a manual fluorimetric method for the assay of acidic alpha-mannosidase activity in bovine plasma. The optimum conditions for the assay of this enzyme were studied. The assay method devised includes the addition of zinc to the substrate, which stimulates activity by approximately twofold, and reduces the optimum substrate concentration. This latter feature affords considerable cost saving in each test. We have also shown that the alpha-mannosidase activity in lithium heparin plasma, EDTA plasma and blood serum is the same whether the plasma/serum is separated from the cells/clot at 6, 20 or 25 hours after sample collection. This has eliminated the previous necessity of having to deliver whole blood samples to the laboratory within 6 hours of collection. Furthermore samples for the supplementary neutrophil assay can now be taken at the same time as those for the plasma test, and both samples forwarded together. The plasma alpha-mannosidase assay is a rapid and reliable screening test for the mannosidosis genotype and for detecting carrier animals. Carrying out this plasma assay in conjunction with the more definitive neutrophil assay provides a reliable method of distinguishing homozygotes and heterozygotes f...Continue Reading

References

May 1, 1983·Australian Veterinary Journal·P J HealyA J Sinclair

Citations

Sep 15, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·P J HealyP Butrej
Aug 1, 1976·Australian Veterinary Journal·P J Healy, A E Cole
Feb 1, 1974·The Biochemical Journal·N C PhillipsR D Jolly
Apr 1, 1973·New Zealand Veterinary Journal·R D JollyR M Greenway
Apr 6, 1968·Nature·S M Snaith, G A Levvy
Oct 1, 1970·New Zealand Veterinary Journal·R D Jolly
Mar 1, 1969·Clinica Chimica Acta; International Journal of Clinical Chemistry·P A Ockerman

Related Concepts

Fluorometry
Heparin
Collection of Blood Specimen for Laboratory Procedure
Lithium Measurement
Bos taurus
Liquaemin
Edetic Acid, Sodium Salt
Zinc
Specimen Collection
Blood Clot

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