An improved immunofluorescence technique for the histological examination of blood vessel tissue.

Acta histochemica
R KittelbergerW E Stehbens

Abstract

Autofluorescence of elastic fibres in blood vessel samples is a common interference with the specific fluorescence of FITC-conjugated antibodies. Counterstaining with eriochrome black T changed the yellow-green colour of elastic fibres to dark red, thus turning a disturbing feature into a useful reference background. A second counterstain, p-phenylenediamine, visualized cell nuclei as an amber colour. To demonstrate the improvement of this staining technique, cryosections from blood vessel samples, derived from control veins, arteries and experimental aneurysms of different ages (15 to 99 month old) in 5 sheep, were stained with antibodies against procollagen III, collagen type IV, laminin, and nidogen. The specific distribution of these connective tissue components could now be related to the location of the elastic fibres and the cells (cell nuclei).

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Citations

Mar 15, 1990·Experientia·R KittelbergerW E Stehbens
Apr 1, 1994·The Journal of Clinical Investigation·R MorishitaV J Dzau
Mar 22, 2007·Journal of the Peripheral Nervous System : JPNS·Markus F StevensPeter Lipfert
Jan 1, 1990·Connective Tissue Research·R KittelbergerN S Greenhill
Nov 29, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W BaschongR H Laeng
Apr 12, 2014·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A Sally DavisJeffery K Taubenberger
Dec 30, 2015·Biomedical Optics Express·Ramla GaryRiccardo Barberi
Aug 25, 1992·Biochimica Et Biophysica Acta·R KittelbergerG J Gibson
Aug 21, 2021·Scientific Reports·Reiji YamazakiNobuhiko Ohno

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