An improved indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antisperm antibodies

American Journal of Reproductive Immunology and Microbiology : AJRIM
R M IngW R Jones

Abstract

An indirect enzyme-linked immunosorbent assay (ELISA) was used for the detection of antisperm antibodies which provides a sensitivity and ease of quantitation that is not available with conventional bioassay systems. A standardized protocol was developed in which washed whole sperm were coated onto polystyrene microelisa plates at a density of 1 X 10(5) sperm per well using a commercially available spray fixative. Urease was employed in the enzyme-antiimmunoglobulin conjugate to minimize nonspecific background reactions. Diluted positive and negative human sera were incubated at 37 degrees C and the results were read on an ELISA auto reader. Using mouse antihuman sperm antisera and sera from selected infertile patients, it was found that the ELISA method was significantly more sensitive than the sperm microimmobilization test and the microtray agglutination test. The results also confirmed that the ELISA detected a different spectrum of sperm antibodies compared with the other two techniques.

References

Mar 1, 1973·Journal of Reproduction and Fertility·W R JonesM D Kaye
Sep 17, 1982·Journal of Immunological Methods·H M ChandlerJ G Hurrell
Jan 28, 1983·Journal of Immunological Methods·S PaulL Mettler
Dec 1, 1982·Fertility and Sterility·R ZanchettaI Mastrogiacomo
Mar 1, 1980·Fertility and Sterility·L A Hill, J K Hampton
Aug 1, 1981·American Journal of Reproductive Immunology : AJRI·S B AckermanR J Swanson
Feb 1, 1982·Biology of Reproduction·D P WolfG G Haas
Jun 24, 1971·FEBS Letters·B K. Van Weemen, A H.W.M. Schuurs

Citations

Sep 1, 1992·American Journal of Reproductive Immunology : AJRI·H Meinertz
Jan 1, 1989·Archives of Andrology·M L WindtT F Kruger
Dec 1, 1986·Asia-Oceania Journal of Obstetrics and Gynaecology·S X WangW R Jones
Dec 5, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·R-Marc PelletierMaría Leiza Vitale
Jul 1, 1986·American Journal of Reproductive Immunology and Microbiology : AJRIM·J C HerrT N Gallien
Sep 1, 1989·American Journal of Reproductive Immunology : AJRI·M ItohK Hojo
Mar 1, 1993·American Journal of Reproductive Immunology : AJRI·R N Fichorova, L S Nakov

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.