An improved liquid chromatography-tandem mass spectrometry method for the quantification of 4-aminobiphenyl DNA adducts in urinary bladder cells and tissues.

Journal of Chromatography. a
Kristen L RandallPaul Vouros

Abstract

Exposure to 4-aminobiphenyl (4-ABP), an environmental and tobacco smoke carcinogen that targets the bladder urothelium, leads to DNA adduct formation and cancer development [1]. Two major analytical challenges in DNA adduct analysis of human samples have been limited sample availability and the need to reach detection limits approaching the part-per-billion threshold. By operating at nano-flow rates and incorporating a capillary analytical column in addition to an online sample enrichment step, we have developed a sensitive and quantitative HPLC-MS/MS method appropriate for the analysis of such samples. This assay for the deoxyguanosine adduct of 4-ABP (dG-C8-4-ABP) gave mass detection limits of 20amol in 1.25microg of DNA (5 adducts in 10(9) nucleosides) with a linear range of 70amol to 70fmol. 4-ABP-exposed human bladder cells and rat bladder tissue were analyzed in triplicate, and higher dose concentrations led to increased numbers of detected adducts. It was subsequently established that sample requirements could be further reduced to 1microg digestions and the equivalent of 250ng DNA per injection for the detection of low levels of dG-C8-4-ABP in a matrix of exfoliated human urothelial cell DNA. This method is appropriate ...Continue Reading

References

Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G TalaskaF F Kadlubar
Mar 1, 1981·Cancer·E C Miller, J A Miller
Oct 1, 1994·Environmental Health Perspectives·P Vineis
Oct 1, 1993·Carcinogenesis·K Hemminki
Jan 26, 1996·International Journal of Cancer. Journal International Du Cancer·P VineisP Strickland
May 1, 1996·Environmental Health Perspectives·D H Phillips
Feb 1, 1997·Analytical Chemistry·Q XueB L Karger
Mar 3, 1998·American Journal of Epidemiology·L Y WangR M Santella
Mar 10, 1998·Journal of Chromatography. a·W A Apruzzese, P Vouros
May 23, 2002·Carcinogenesis·Luisa AiroldiPaolo Vineis
Oct 12, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hasan Koc, James A Swenberg
Sep 16, 2003·Chemical Research in Toxicology·Robert J TureskyFred F Kadlubar
Mar 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Robert J Turesky, Paul Vouros
Oct 19, 2004·Seminars in Cancer Biology·Gerald N WoganLawrence A Loeb
Jan 22, 2005·Nature Reviews. Cancer·Andreas Luch
Aug 1, 2006·Journal of the American Society for Mass Spectrometry·Qingrong ZhangDaniele Fabris
Aug 24, 2006·Carcinogenesis·Beatriz ZayasGerald N Wogan
Jan 15, 2008·Chemical Research in Toxicology·Yinsheng Wang
Mar 4, 2008·Cancer Research·Rex MundayYuesheng Zhang
Jun 19, 2008·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Susan PetersRoel Vermeulen
May 16, 2009·Chemical Research in Toxicology·Jean-Marie FedeRobert J Turesky

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Citations

Dec 1, 1995·Mutation Research·E RozengurtA M Valverde
Aug 17, 1996·Mutation Research·M Sorsa, D Anderson
Jan 1, 1990·Advances in Enzyme Regulation·L RaptisJ F Whitfield
Dec 1, 1993·Mutation Research·D M SalvadoriA T Natarajan
Oct 2, 2001·Pharmacology & Therapeutics·M LeszczynieckaP B Fisher
Apr 22, 1999·Immunology Today·J LloberasA Celada
Nov 20, 1998·Journal of Photochemistry and Photobiology. B, Biology·S Legrand-PoelsJ Piette
Feb 28, 2013·Chemical Reviews·Natalia TretyakovaSrikanth Kotapati
Jul 26, 2012·Chemical Research in Toxicology·Natalia TretyakovaGregory Janis
Nov 27, 2015·Journal of Chromatography. a·Joshua J KlaenePaul Vouros
Sep 11, 2012·International Journal of Cancer. Journal International Du Cancer·David H Phillips, Stan Venitt
Nov 29, 2013·Mass Spectrometry Reviews·Ed Dudley, Liz Bond
Sep 2, 2016·Current Protocols in Nucleic Acid Chemistry·Jingshu Guo, Robert J Turesky
Jul 13, 2018·Analytical Chemistry·Byeong Hwa YunRobert J Turesky

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