An improved method for Agrobacterium rhizogenes -mediated transformation of tomato suitable for the study of arbuscular mycorrhizal symbiosis

Plant Methods
Tania Ho-PlágaroJosé Manuel García-Garrido

Abstract

Solanum lycopersicum, an economically important crop grown worldwide, has been used as a model for the study of arbuscular mycorrhizal (AM) symbiosis in non-legume plants for several years and several cDNA array hybridization studies have revealed specific transcriptomic profiles of mycorrhizal tomato roots. However, a method to easily screen candidate genes which could play an important role during tomato mycorrhization is required. We have developed an optimized procedure for composite tomato plant obtaining achieved through Agrobacterium rhizogenes-mediated transformation. This protocol involves the unusual in vitro culture of composite plants between two filter papers placed on the culture media. In addition, we show that DsRed is an appropriate molecular marker for the precise selection of cotransformed tomato hairy roots. S. lycopersicum composite plant hairy roots appear to be colonized by the AM fungus Rhizophagus irregularis in a manner similar to that of normal roots, and a modified construct useful for localizing the expression of promoters putatively associated with mycorrhization was developed and tested. In this study, we present an easy, fast and low-cost procedure to study AM symbiosis in tomato roots.

References

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May 7, 2002·Trends in Plant Science·Mansour KarimiAnn Depicker
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Mar 30, 2016·Plant Physiology·Igor S KryvoruchkoMichael K Udvardi
Sep 8, 2016·Frontiers in Plant Science·José A Martín-RodríguezJosé M García-Garrido

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Citations

Jan 13, 2019·Plants·Angelo De PaolisSofia Caretto
Sep 24, 2020·The New Phytologist·Tania Ho-PlágaroJosé Manuel García-Garrido
Aug 8, 2021·International Journal of Molecular Sciences·Yvann BourigaultXavier Latour
Oct 31, 2021·Molecular Biology Reports·Jyotsana TilgamAnil Kumar Saxena

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Datasets Mentioned

BETA
X14449

Methods Mentioned

BETA
transgenic
PCR

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