An improved method for primary culture of rat podocytes

Kidney International
K KatsuyaT Yamamoto

Abstract

A gentle method to isolate glomeruli simply by cutting renal cortices without forced sieving was devised in our previous study of primary podocyte culture. Yields of glomeruli isolated by this method, however, were too small to perform subculture or biological assays. In the present study, we tried an isolation method with magnetic beads and collagenase to increase the yields. Rat kidneys were perfused with magnetic particles. Renal cortices were digested with collagenase and filtered. Utilizing magnetic particles trapped within glomeruli, glomeruli were collected by attractive power of a magnet and cultured. The number of glomeruli isolated from one adult rat was more than 20,000 and the purity was more than 97%. About half of them were attached to culture dishes and exhibited cellular outgrowths, which were identified as podocytes by their distinct staining for podocyte markers. After 3 days of primary culture, the cellular outgrowths were subcultured. Approximately 60 podocytes were obtained per attached glomerulus. Their significant expression of podocytes markers was demonstrated by immunostaining and quantitative reverse transcriptase-polymerase chain reaction. The isolation method with magnetic beads and collagenase prov...Continue Reading

References

May 1, 1997·Journal of the American Society of Nephrology : JASN·P MundelW Kriz
Dec 16, 1998·Kidney International·Y Ishikawa, M Kitamura
Jul 18, 2001·Cell and Tissue Research·E YaoitaT Yamamoto
Feb 22, 2002·Journal of the American Society of Nephrology : JASN·Moin A SaleemPeter Mundel
Sep 6, 2002·The American Journal of Pathology·Minoru TakemotoChrister Betsholtz
Nov 5, 2002·The American Journal of Pathology·Eishin YaoitaTadashi Yamamoto

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Citations

Apr 12, 2012·The Journal of Biological Chemistry·Sandeep K MallipattuJohn C He
Jun 10, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Ayumi MurakamiKiyotaka Nagahama
May 18, 2012·Nephrology·Lan NiPeter W Mathieson
May 7, 2010·American Journal of Physiology. Cell Physiology·Yoshifusa AbeJeffrey B Kopp
Jul 30, 2010·American Journal of Physiology. Renal Physiology·Toru SakairiJeffrey B Kopp
Dec 4, 2009·American Journal of Physiology. Renal Physiology·Toru SakairiJeffrey B Kopp
Nov 29, 2012·The Journal of Clinical Investigation·Keita SodaShuta Ishibe
Jun 5, 2010·Journal of the American Society of Nephrology : JASN·Hong MaShuta Ishibe
Jul 2, 2011·Journal of the American Society of Nephrology : JASN·Xue-Yuan BaiXiang-Mei Chen
Mar 5, 2011·Nihon Rinshō Men'eki Gakkai kaishi = Japanese journal of clinical immunology·Hiroshi Kajiyama
Apr 25, 2015·Proteomics. Clinical Applications·Markus M RinschenTrairak Pisitkun
Jan 18, 2014·Nephrology·Eishin YaoitaTadashi Yamamoto
Jan 1, 2012·The Tohoku Journal of Experimental Medicine·Patrick TaeJoon HwangChan-Wha Kim
Apr 26, 2011·The American Journal of Pathology·Chunhua ZhuAihua Zhang
Feb 18, 2015·The Journal of Clinical Investigation·Sandeep K MallipattuJohn C He
Feb 9, 2016·The Journal of Veterinary Medical Science·Go SugaharaKinji Shirota
Jun 12, 2016·Journal of the American Society of Nephrology : JASN·Sandeep K MallipattuJohn C He
Nov 11, 2016·International Journal of Nephrology·Saeed Yadranji Aghdam, Ali Mahmoudpour
Jul 1, 2016·PloS One·Junichi TsuchidaKiyoshi Mori
Sep 18, 2018·PloS One·Caroline RauchAnja Wilmes
Oct 11, 2017·Journal of the American Society of Nephrology : JASN·Sylvia CechovaThu H Le
Aug 26, 2018·Journal of the American Society of Nephrology : JASN·Yiqing GuoSandeep K Mallipattu
Aug 10, 2007·Journal of the American Society of Nephrology : JASN·Aya NakajoKunimasa Yan
Apr 21, 2017·Journal of the American Society of Nephrology : JASN·Tamadher A AlghamdiAndrew Advani
Jan 29, 2020·Cell and Tissue Research·Tomizo OyamaHidehiko Fujinaka
Feb 11, 2011·American Journal of Physiology. Renal Physiology·Sahoko SekiguchiMitsuyasu Itoh
Sep 13, 2019·The Journal of Biological Chemistry·Rajkishor NishadAnil K Pasupulati
Dec 22, 2020·The Journal of Experimental Medicine·Shuai HuZhihong Liu
Jul 27, 2021·Cell and Tissue Research·Eishin YaoitaHidehiko Fujinaka
Sep 12, 2017·Kidney International·Eishin YaoitaHidehiko Fujinaka

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