An improved method for the determination of 5-phosphoribosyl 1-pyrophosphate.

Clinica Chimica Acta; International Journal of Clinical Chemistry
V MicheliR Marcolongo

Abstract

A spectrophotometric method for the determination of 5-phosphoribosyl 1-pyrophosphate (PRPP) is presented which shows several advantages in comparison to the radiochemical techniques, such as a relatively simple, rapid and less expensive procedure. This technique has been used to evaluate PRPP content in erythrocytes, leukocytes and lymphocytes of normal subjects and individuals with partial hypoxanthine guanine phosphoribosyltransferase (EC 2.4.2.8) deficiency. The results obtained proved to be completely reliable in both groups of subjects examined, with values of PRPP similar to those observed by radiochemical techniques.

References

Mar 1, 1971·Annals of Internal Medicine·I H Fox, W N Kelley
Jan 1, 1971·Annual Review of Biochemistry·A W Murray
May 1, 1974·The American Journal of Medicine·J B Wyngaarden
Aug 1, 1973·The American Journal of Medicine·M A BeckerJ E Seegmiller
Sep 1, 1962·The Journal of Clinical Investigation·O W JONESJ B WYNGAARDEN

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Citations

Jan 1, 1978·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·W J Tax, J H Veerkamp
Sep 18, 2004·Journal of Bacteriology·Michael W Finn, F Robert Tabita
Jul 15, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·W J Tax, J H Veerkamp

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