An improved method for the isolation of type II and Clara cells from mice

In Vitro Cellular & Developmental Biology. Animal
S A BelinskyN F Johnson

Abstract

Identifying the causal events and temporal aspects of lung cancer development requires the ability to isolate target and nontarget cells for comparative analyses. Current methodology can either isolate only one pure specific cell population from a lung or multiple cell types at lower purity. Previous studies in our laboratory have identified the alveolar type II cell as the progenitor cell for tumor development in the A/J mouse. The purpose of this study was to develop new protocols for the isolation and culture of type II and Clara cells from the mouse lung. Both type II and Clara cells were obtained in high purity using a sequential centrifugal elutriation protocol. In the first elutriation, cell fractions were collected using a Standard chamber. The type II and Clara cell fractions were then elutriated separately (two different separations) using a Sanderson chamber. The final purity of the type II and Clara cell preparations was 73% and 76%, respectively. Colonies of 4 to 20 Clara cells exhibiting epithelial morphology were evident 1 wk after plating in low serum medium. The growth of type II cells required the addition of bronchioalveolar lavage fluid and acidic fibroblast growth factor to the medium. The isolation of viab...Continue Reading

References

Jun 1, 1990·Cell·E R Fearon, B Vogelstein
Feb 1, 1989·The American Review of Respiratory Disease·C C LeslieR J Mason
Jul 1, 1989·Experimental Lung Research·S R WalkerR J Mason
Dec 1, 1987·Canadian Journal of Physiology and Pharmacology·T E MasseyP G Forkert
Nov 1, 1980·In Vitro·T R Devereux, J R Fouts
Jan 1, 1981·Methods in Enzymology·T R Devereux, J R Fouts
Jan 1, 1995·Virchows Archiv : an International Journal of Pathology·F C SchmittM Lacerda
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·R W WisemanP Söderkvist
Jul 1, 1993·American Journal of Respiratory Cell and Molecular Biology·C C LeslieJ M Shannon

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Citations

Jun 9, 2005·Experimental and Molecular Pathology·Renald A Blundell, David J Harrison
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·S A BelinskyJ P Issa
Jan 9, 2008·Respiratory Research·Jeffrey J AtkinsonRobert M Senior
Apr 1, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Tanja HansenJürgen Borlak
May 29, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Arnon ElizurRobert M Senior
Jun 17, 2016·Chronobiology International·Michael H SmolenskyFrancesco Portaluppi
Jun 13, 2019·Respiratory Research·Guo-Peng LiangYan Kang
Apr 11, 2006·Molecular Cancer Research : MCR·Brian R VuillemenotSteven A Belinsky
Mar 1, 1997·Molecular and Cellular Biology·D S SwaffordS A Belinsky

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