PMID: 7017346Jan 1, 1981Paper

An improved method for two-dimensional gel-electrophoresis: analysis of mutationally altered ribosomal proteins of Escherichia coli

Molecular & General Genetics : MGG
D GeylK Isono

Abstract

An improved method for the two-dimensional electrophoretic analysis of ribosomal proteins on acrylamide gel slabs has been developed by combining the procedures for the first dimension of Mets and Bogorad (1974) and for the second dimension of Kaltschmidt and Wittmann (1970) and by introducing several modifications. Ribosomal proteins of various Escherichia coli mutants have been analyzed by the new method. Advantages are that (1) it requires only small amounts of protein (100-200 micrograms 70S ribosomal proteins), (2) reproducibility is very high, and (3) it makes it easier to identify mutational alterations in proteins S10, L4, L10, and L21 which hardly migrate out of the sample gel with our previous electrophoresis procedure. Furthermore, the new method can be nicely adapted to analysis of the ribosomal proteins from other organisms, such as Bacilli or yeast.

References

Jan 20, 1977·Biochimica Et Biophysica Acta·A Kyriakopoulos, A R Subramanian
Apr 29, 1977·Molecular & General Genetics : MGG·K IsonoS Isono
Sep 20, 1978·Molecular & General Genetics : MGG·E R Dabbs
Dec 22, 1976·Molecular & General Genetics : MGG·E R Dabbs, H G Wittman
Jan 1, 1974·Molecular & General Genetics : MGG·H G Wittmann, G Stöffler
Jan 1, 1980·Molecular & General Genetics : MGG·H HummelA Böck

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Citations

Jul 1, 1990·Electrophoresis·T Choli, B Wittmann-Liebold
Jan 1, 1981·Molecular & General Genetics : MGG·A Böck
Jan 1, 1983·Molecular & General Genetics : MGG·H Hummel, A Böck
Dec 10, 1999·Folia Microbiologica·K MikulíkS Bezousková
Feb 1, 1995·The International Journal of Biochemistry & Cell Biology·C A PanagiotidisE S Canellakis
Jun 24, 2003·Journal of Molecular Biology·Natalya Van Dyke, Emanuel J Murgola
Aug 30, 2003·Journal of Molecular Biology·Fotini LeontiadouTheodora Choli-Papadopoulou
Jul 27, 2001·Biochimica Et Biophysica Acta·R WillumeitK H Nierhaus
Feb 6, 1998·Biochimie·A B UlitinI N Serdyuk
Feb 13, 2001·The Journal of Peptide Research : Official Journal of the American Peptide Society·R I Boysen, M T Hearn
Jun 13, 2001·Molecular Microbiology·J Usary, W S Champney
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M P CaulfieldB D Davis
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·R R SamahaH F Noller
Aug 15, 1983·European Journal of Biochemistry·F J van de VenC W Hilbers
Dec 3, 1984·European Journal of Biochemistry·K MikulíkA Jiránová
Dec 2, 1985·European Journal of Biochemistry·M KimuraK Watanabe

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