An improved microfluidics approach for monitoring real-time interaction profiles of ultrafast molecular recognition

The Review of Scientific Instruments
Subrata BatabyalSamir Kumar Pal

Abstract

Our study illustrates the development of a microfluidics (MF) platform combining fluorescence microscopy and femtosecond/picosecond-resolved spectroscopy to investigate ultrafast chemical processes in liquid-phase diffusion-controlled reactions. By controlling the flow rates of two reactants in a specially designed MF chip, sub-100 ns time resolution for the exploration of chemical intermediates of the reaction in the MF channel has been achieved. Our system clearly rules out the possibility of formation of any intermediate reaction product in a so-called fast ionic reaction between sodium hydroxide and phenolphthalein, and reveals a microsecond time scale associated with the formation of the reaction product. We have also used the developed system for the investigation of intermediate states in the molecular recognition of various macromolecular self-assemblies (micelles) and genomic DNA by small organic ligands (Hoechst 33258 and ethidium bromide). We propose our MF-based system to be an alternative to the existing millisecond-resolved "stopped-flow" technique for a broad range of time-resolved (sub-100 ns to minutes) experiments on complex chemical∕biological systems.

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Citations

May 31, 2014·IEEE Transactions on Biomedical Circuits and Systems·Calliope-Louisa SotiropoulouSpyridon Nikolaidis
Sep 15, 2015·Chemistry : a European Journal·Susobhan ChoudhurySamir Kumar Pal

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Methods Mentioned

BETA
fluorescence
fluorescence microscopy
chip

Software Mentioned

mathop
ANALYSIS

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