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An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases

Analytical Biochemistry

May 24, 2011

Dongming LanAnlong Xu

Abstract

Proteins fused to the elastin-like polypeptide (ELP) tag can be selectively separated from crude cell extract without chromatography. To avoid the interference of the ELP tag on properties of the target protein, it is necessary to remove the ELP tag from target protein by protease diges...read more

Mentioned in this Paper

ELN gene
Chimeric Proteins, Recombinant
Peptide Hydrolases
Chromatography
One-Step Dentin Bonding System
Cytokinesis of the Fertilized Ovum
Endopeptidases
Proteolytic Enzyme
Centrifugation
Cleaved Cell
Paper Details
References
  • References13
  • Citations5
12
  • References13
  • Citations5
1

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An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases

Analytical Biochemistry

May 24, 2011

Dongming LanAnlong Xu

PMID: 21600185

DOI: 10.1016/j.ab.2011.04.034

Abstract

Proteins fused to the elastin-like polypeptide (ELP) tag can be selectively separated from crude cell extract without chromatography. To avoid the interference of the ELP tag on properties of the target protein, it is necessary to remove the ELP tag from target protein by protease diges...read more

Mentioned in this Paper

ELN gene
Chimeric Proteins, Recombinant
Peptide Hydrolases
Chromatography
One-Step Dentin Bonding System
Cytokinesis of the Fertilized Ovum
Endopeptidases
Proteolytic Enzyme
Centrifugation
Cleaved Cell

Similar Papers Found In These Feeds

Oxygenation & Hydroxylation

Oxygenation is a chemical production that introduces oxygen atom into an organic compound. Hydroxylation is a chemical process that introduces a hydroxyl group (-OH) into an organic compound. In biochemistry, hydroxylation reactions are often facilitated by enzymes called hydroxylases. Cytochrome P450 (CYP) enzymes are expressed mainly in the liver and are active in mono-oxygenation and hydroxylation of various xenobiotics, including drugs and alcohols, as well as that of endogenous compounds such as steroids, bile acids, prostaglandins, leukotrienes and biogenic amines. Here is the latest research.

Related Papers

Current Protocols in Protein Science

Elastin-like polypeptides as a purification tag for recombinant proteins

Current Protocols in Protein ScienceSeptember 4, 2010
Wafa HassounehAshutosh Chilkoti
Protein Science : a Publication of the Protein Society

Fusion order controls expression level and activity of elastin-like polypeptide fusion proteins

Protein Science : a Publication of the Protein SocietyJune 18, 2009
Trine ChristensenAshutosh Chilkoti
Paper Details
References
  • References13
  • Citations5
12
  • References13
  • Citations5
1
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