An improved nonlinear model describing the hepatic pharmacokinetics of digoxin: evidence for two functionally different uptake systems and saturable binding.

Pharmaceutical Research
Michael WeissM S Roberts

Abstract

To develop a semi-distributed liver model for the evaluation of saturable sinusoidal uptake and binding kinetics of the Oatp1a4 substrate digoxin. In the perfused rat liver, two successive digoxin doses of 42 and 125 microg were administered, and the outflow concentration was determined by LC/MS/MS. [14C]-sucrose was used as vascular reference. The data were analyzed simultaneously by a population approach using sucrose to determine the sinusoidal mixing of digoxin. The results suggest the existence of a high-affinity, low-capacity system, and a low-affinity, high-capacity system for sinusoidal uptake with apparent Michaelis constants (K(M)) of 0.24 and 332 microg/ml, respectively. Incorporation of saturable sinusoidal binding of digoxin considerably improved the fit, and the parameter estimates were consistent with those of binding to hepatic Na,K-ATPase. Simpler models that neglect the concentration gradient in flow direction failed to describe the outflow data in the high dose range. The semi-distributed liver model with saturable uptake should be useful for a functional characterization of transporters in the in situ rat liver.

References

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Jun 25, 2008·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Michael WeissMichael S Roberts
Aug 20, 2009·Current Drug Metabolism·Peng LiMichael S Roberts
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May 7, 2010·Clinical and Translational Science·Marianthi G LerapetritouLoannis P Androulakis

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Citations

Nov 26, 2010·Journal of Pharmacokinetics and Pharmacodynamics·Michael S Roberts
Jun 25, 2013·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Michael WeissMichael S Roberts
Jul 4, 2012·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Michael WeissMichael S Roberts
Oct 30, 2013·Journal of Clinical Pharmacology·Ahmed M Nader, David R Foster
Jul 24, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Peng LiMichael S Roberts
Jun 18, 2016·Journal of Biophotonics·Haolu WangMichael S Roberts

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