An improved preparation of antibody-coated polystyrene beads for sandwich enzyme immunoassay

Journal of Immunoassay
E IshikawaH Ogawa

Abstract

An improved preparation of antibody-coated polystyrene beads for sandwich enzyme immunoassay of human thyroid-stimulating hormone (TSH) was described. Rabbit anti-TSH IgG was purified by eluting at pH 2.5 from a TSH-Sepharose column, diluted 3 or 9 fold with normal rabbit IgG and used for coating polystyrene beads by physical adsorption. In a sandwich enzyme immunoassay of TSH using rabbit (anti-TSH) Fab'-beta-D-galactosidase conjugate, beta-D-galactosidase activities specifically bound to thus prepared polystyrene beads in the presence of TSH was 2.8-6.3 fold higher than those bound to polystyrene beads coated with anti-TSH IgG before purification. A similar effect was observed when guinea pig anti-pork insulin IgG, rabbit (anti-human IgE) IgG and goat (anti-human IgE) IgG were treated at pH 2.5. This improvement may be based on a conformational change of Fc in IgG molecule which was caused by the treatment at pH 2.5. Other sandwich immunoassays such as fluoro- and radio-immunoassays may also be improved in the same way.

References

Jan 1, 1979·Scandinavian Journal of Immunology·S YoshitakeE Ishikawa
Jul 16, 1979·Clinica Chimica Acta; International Journal of Clinical Chemistry·Y EndoE Ishikawa
Jan 1, 1978·Vox Sanguinis·H AdachiE Ishikawa

❮ Previous
Next ❯

Citations

Dec 9, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·M ImagawaY Tsunetoshi
Dec 23, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·M ImagawaK Nakajima
Dec 15, 1989·Clinica Chimica Acta; International Journal of Clinical Chemistry·E IshikawaT Kohno
Dec 20, 1989·Journal of Immunological Methods·J N LinI N Chang
Jun 24, 1992·Journal of Immunological Methods·J E ButlerL A Cantarero
Aug 10, 1992·Journal of Immunological Methods·R van ErpT C Gribnau
Nov 1, 1996·Journal of Immunoassay·A CuvelierR Sesboüé
Jan 4, 1993·Journal of Immunological Methods·V OlivieriF Santoro
Aug 1, 1987·Clinical and Experimental Pharmacology & Physiology·J G McDougallR D Wright
Jan 1, 1994·Journal of Biomaterials Science. Polymer Edition·J L Ortega Vinuesa, R Hidalgo-Alvarez
Sep 6, 2013·Chemical Reviews·Jose RamosRoque Hidalgo-Alvarez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.