PMID: 42342Sep 1, 1979

An improved radioenzymatic assay for histamine in human plasma, whole blood, urine, and gastric juice

Annals of Clinical Biochemistry
C BruceA Westwood


A radioenzymatic method suitable for the assay of histamine in human blood, urine, plasma, and gastric juice is described. It differs from earlier methods by use of a histamine methyltransferase preparation from pig brain, of high activity tritiated S-adenosylmethionine, and of a heat precipitation step to reduce the previously noted interference from plasma constituents. The method is simpler than those requiring solvent extraction and concentration of histamine, gives recoveries in the range 80-120%, and so sliminates the need for internal standardisation. The method is sensitive and precise with coefficients of variation for blood, urine, and plasma of 5%, 6%, and 13% respectively. The mean +/- standard deviation for normal human plasma histamine is 5 +/- 4 nmol/l, for whole blood 559 +/- 193 nmol/l, and for urine 229 +/- 128 nmol/24h.


Sep 1, 1976·American Journal of Public Health·K A BucherH N Kirkman
Aug 1, 1967·Biochemical Pharmacology·K J Netter, K Bodenschatz
Jun 1, 1972·Hoppe-Seyler's Zeitschrift für physiologische Chemie·W LorenzM Hutzel
Sep 1, 1973·Hoppe-Seyler's Zeitschrift für physiologische Chemie·H BarthI Niemeyer
Nov 1, 1968·Biochemical Pharmacology·H GrahamO H Lowry
Mar 1, 1972·Clinica Chimica Acta; International Journal of Clinical Chemistry·M A BeavenZ Horáková
Aug 1, 1957·Biochimica Et Biophysica Acta·R KAPELLER-ADLER, B IGGO
Jul 15, 1960·Acta Physiologica Scandinavica·K M LINDAHL
Jul 1, 1962·Clinica Chimica Acta; International Journal of Clinical Chemistry·J A OATESA SJOERDSMA


Oct 9, 1998·Clinica Chimica Acta; International Journal of Clinical Chemistry·C Bruce, W H Taylor
Sep 1, 1982·Klinische Wochenschrift·J K KhandelwalJ P Green
Jul 1, 1990·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·E OostingW Lorenz

Related Concepts

Gastric Juice
Histamine N-Methyltransferase
Hydrogen-Ion Concentration

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