An improved screening method for microorganisms able to convert crude glycerol to 1,3-propanediol and to tolerate high product concentrations

Applied Microbiology and Biotechnology
Anne Katrin RingelKlaus-Dieter Vorlop

Abstract

A new screening method was developed and established to find high-performance bacteria for the conversion of crude glycerol to 1,3-propanediol. Three soil samples from palm oil-rich habitats were investigated using crude glycerol of a German biodiesel plant. Nine promising 1,3-propanediol producers could be found. Because of a special pH buffer system, a fast evaluation on microscale and high 1,3-propanediol concentrations up to 40 g L⁻¹ could be achieved. Three strains demonstrated very high product tolerance and were identified as Clostridium butyricum. Two strains, AKR91b and AKR102a, grew and produced 1,3-propanediol in the presence of 60 g L⁻¹ initial 1,3-propanediol, the strain AKR92a even in the presence of 77 g L⁻¹ 1,3-propanediol. The strains AKR91b and AKR102a tolerated up to 150 g L⁻¹ crude glycerol and produced 80% of the 1,3-propanediol attained from pure glycerol of the same concentration. Further criteria for the choice of a production strain were the pathogenicity (risk class), ability to grow on low-cost media, e.g., with less yeast extract, and robustness, e.g., process stability after several bioconversions. Overall, the strain C. butyricum AKR102a was chosen for further process optimization and scale-up due ...Continue Reading

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Citations

Oct 6, 2011·Applied Microbiology and Biotechnology·Erik WilkensKlaus-Dieter Vorlop
Feb 22, 2014·BMC Microbiology·Daria Szymanowska-Powałowska, Wojciech Białas
Jun 12, 2013·Bioprocess and Biosystems Engineering·Donna Dietz, An-Ping Zeng
Jul 4, 2012·Journal of Industrial Microbiology & Biotechnology·Pinki Anand, Rajendra Kumar Saxena
Nov 28, 2012·Trends in Biotechnology·James M Clomburg, Ramon Gonzalez
Mar 11, 2016·Applied Biochemistry and Biotechnology·Xianghui QiZheng Cao
Jan 21, 2015·Critical Reviews in Biotechnology·Mine Gungormusler-YilmazNuri Azbar
Dec 20, 2014·Applied Microbiology and Biotechnology·Daria Szymanowska-Powałowska, Piotr Kubiak
Oct 15, 2013·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Kerisha RaghunandanSuren Singh
Oct 1, 2020·Applied Microbiology and Biotechnology·Xiao-Li WangZhi-Long Xiu
Dec 22, 2019·Applied Biochemistry and Biotechnology·Wei-Chuan ChenYu-Hong Wei
May 26, 2017·Applied Biochemistry and Biotechnology·Yong-Juan LiuZhong-Gui Mao

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