PMID: 2509566Oct 24, 1989Paper

An improved spectrofluorometric assay for quantitating yeast phagocytosis in cultures of murine peritoneal macrophages

Journal of Immunological Methods
R L Ragsdale, R J Grasso

Abstract

An accurate means by which to quantitate phagocytosis in murine resident peritoneal macrophages was developed by improving upon existing methods for measuring this important cellular function. Heat-killed Saccharomyces cerevisiae were conjugated to fluorescein isothiocyanate (FITC) and added to macrophage cultures. Following removal of uningested yeast, the macrophages were lysed and the fluorescence associated with the lysates was quantitated. The SEM were rarely +/- 10%. The improved assay was utilized to demonstrate the suppression of yeast phagocytosis by dexamethasone as measured by our radiometric assay. The spectrofluorometric assay produced results similar to those observed when the radiometric assay was employed to determine steroid induced suppression of yeast phagocytosis. However, the improved spectrofluorometric assay is more accurate, reliable, easier to perform, cost and time efficient, and a much safer method for quantitating yeast phagocytosis.

References

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Aug 9, 1988·Journal of Immunological Methods·J A Oben, J C Foreman
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Citations

Jun 4, 1993·Journal of Immunological Methods·C P WanB H Lau
Sep 14, 1994·Journal of Immunological Methods·I FraserS Gordon
Sep 14, 1994·Journal of Immunological Methods·N Plasman, B Vray
Mar 1, 1993·Developmental and Comparative Immunology·J R FowlesN I Kerkvliet
Jan 24, 1998·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·J R FowlesN I Kerkvliet
Apr 6, 2007·Biological & Pharmaceutical Bulletin·Mitsuaki HayashiMasakatsu Tezuka
Apr 28, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Uma SinghAndrés López Bernal
May 17, 2000·The Journal of Cell Biology·T KanazawaY Kozutsumi
Mar 11, 2015·Journal of Clinical and Experimental Hepatology·Abdel Motaal M AhmedRoger Williams

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