An improved technique for isolating codominant compound microsatellite markers.

Journal of Plant Research
Chunlan LianTaizo Hogetsu

Abstract

An approach for developing codominant polymorphic markers (compound microsatellite (SSR) markers), with substantial time and cost savings, is introduced in this paper. In this technique, fragments flanked by a compound SSR sequence at one end were amplified from the constructed DNA library using compound SSR primer (AC)6(AG)5 or (TC)6(AC)5 and an adaptor primer for the suppression-PCR. A locus-specific primer was designed from the sequence flanking the compound SSR. The primer pairs of the locus-specific and compound SSR primers were used as a compound SSR marker. Because only one locus-specific primer was needed for design of each marker and only a common compound SSR primer was needed as the fluorescence-labeled primer for analyzing all the compound SSR markers, this approach substantially reduced the cost of developing codominant markers and analyzing their polymorphism. We have demonstrated this technique for Dendropanax trifidus and easily developed 11 codominant markers with high polymorphism for D. trifidus. Use of the technique for successful isolation of codominant compound SSR markers for several other plant species is currently in progress.

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Citations

Jul 16, 2009·Journal of Plant Research·Sayaka Nakagawa, Motomi Ito
May 1, 2008·Molecular Ecology Resources·Naoko Isomura, Michio Hidaka
Jan 1, 2013·Applications in Plant Sciences·Asuka YamashiroYoichi Tateishi
Sep 10, 2014·Applications in Plant Sciences·Satomi SakazonoHiroshi Okubo
Mar 24, 2015·Applications in Plant Sciences·Shinichiro KameokaHiroaki Setoguchi
Sep 10, 2014·Applications in Plant Sciences·Satoka Yamauchi, Takanori Ohsako
Feb 5, 2015·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Kit-Siong LiewAbdullah Julaihi
Feb 7, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qi-Fang GengShu-Qing An
Jan 26, 2012·American Journal of Botany·Na YuanYing-Xiong Qiu
Jan 10, 2012·American Journal of Botany·Y Kameyama
May 28, 2011·American Journal of Botany·Sheng-Nan ZhaiYing-Xiong Qiu
Jul 28, 2012·American Journal of Botany·Xin-Shuai QiYing-Xiong Qiu

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