An Improved Ultrasensitive Enzyme-Linked Immunosorbent Assay Using Hydrangea-Like Antibody-Enzyme-Inorganic Three-in-One Nanocomposites

ACS Applied Materials & Interfaces
Tianxiang WeiZhihui Dai

Abstract

Protein-inorganic nanoflowers, composed of protein and copper(II) phosphate (Cu3(PO4)2), have recently grabbed people's attention. Because the synthetic method requires no organic solvent and because of the distinct hierarchical nanostructure, protein-inorganic nanoflowers display enhanced catalytic activity and stability and would be a promising tool in biocatalytical processes and biological and biomedical fields. In this work, we first coimmobilized the enzyme, antibody, and Cu3(PO4)2 into a three-in-one hybrid protein-inorganic nanoflower to enable it to possess dual functions: (1) the antibody portion retains the ability to specifically capture the corresponding antigen; (2) the nanoflower has enhanced enzymatic activity and stability to produce an amplified signal. The prepared antibody-enzyme-inorganic nanoflower was first applied in an enzyme-linked immunosorbent assay to serve as a novel enzyme-labeled antibody for Escherichia coli O157:H7 (E. coli O157:H7) determination. The detection limit is 60 CFU L(-1), which is far superior to commercial ELISA systems. The three-in-one antibody (anti-E. coli O157:H7 antibody)-enzyme (horseradish peroxidase)-inorganic (Cu3(PO4)2) nanoflower has some advantages over commercial enzy...Continue Reading

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Citations

Sep 13, 2016·Biosensors & Bioelectronics·Evgeni Eltzov, Robert S Marks
Aug 8, 2018·Analytical and Bioanalytical Chemistry·Chengke WangQingqing Wang
Mar 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhixian LeiZian Lin
Mar 12, 2021·Critical Reviews in Analytical Chemistry·Indra Gandi SubramaniNorani Muti Mohamed
Mar 13, 2017·ACS Biomaterials Science & Engineering·Shuai ChenJian-Hua Wang
Jul 12, 2018·ACS Applied Materials & Interfaces·Caihong WangHongliang Tan
Oct 30, 2021·Analytical Chemistry·Parthena ChortiDionysios C Christodouleas

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