An in situ antimicrobial susceptibility testing method based on in vivo measurements of chlorophyll α fluorescence

Journal of Microbiological Methods
Nikolaos S HeliopoulosKostas Stamatakis

Abstract

Up to now antimicrobial susceptibility testing (AST) methods are indirect and generally involve the manual counting of bacterial colonies following the extraction of microorganisms from the surface under study and their inoculation in a separate procedure. In this work, an in situ, direct and instrumental method for the evaluation and assessment of antibacterial properties of materials and surfaces is proposed. Instead of indirectly determining antibacterial activity using the typical gram(-) test organisms with the subsequent manual colony count or inhibition zone measurement, the proposed procedure, employs photosynthetic gram(-) cyanobacteria deposited directly onto the surface under study and assesses cell proliferation and viability by a quick, accurate and reproducible instrumental chlorophyll fluorescence spectrophotometric technique. In contrast with existing methods of determination of antibacterial properties, it produces high resolution and quantitative results and is so versatile that it could be used to evaluate the antibacterial properties of any compound (organic, inorganic, natural or man-made) under any experimental conditions, depending on the targeted application.

References

Dec 15, 1986·Biochemical and Biophysical Research Communications·B D HsuR L Pan
Mar 1, 1973·Antimicrobial Agents and Chemotherapy·T D Wilkins, T Thiel
Aug 1, 1959·A.M.A. Archives of Internal Medicine·A W BAUERW M KIRBY
Aug 1, 2007·Photosynthesis Research·George C PapageorgiouKostas Stamatakis
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Lee Macomber, James A Imlay
Feb 23, 2010·Journal of Microbiological Methods·Anja KlancnikSonja Smole Mozina
Jan 5, 2011·Applied and Environmental Microbiology·Gregor GrassMarc Solioz
Jun 13, 2014·Biological Trace Element Research·Chunnuan DengDaoyong Zhang

❮ Previous
Next ❯

Citations

Dec 17, 2015·Journal of Microbiological Methods·Nikolaos S HeliopoulosKostas Stamatakis
Sep 30, 2016·ACS Applied Materials & Interfaces·Anna PerdikakiGeorgios N Karanikolos
Oct 24, 2017·ACS Applied Materials & Interfaces·Kosmas EllinasAngeliki Tserepi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.