An in vitro assay for T lymphocyte progenitors (CFU-pre T)

Journal of Supramolecular Structure
B R Acuff, J J Cohen

Abstract

Thy-1.2 negative progenitors give rise to Thy-1.2 positive colony cells when mouse bone marrow is cultured in vitro. The bone marrow cells are immobilized in a viscous medium containing methyl cellulose; discrete colonies are identifiable at 2 days and contain 30-60 cells by day 3 of culture. Colonies are tightly packed spheres (raspberries) and grow suspended in the gel. Growth of the raspberry colonies is absolutely dependent upon the presence of the appropriate serum (horse or human; not fetal calf) and conditioned medium from pokeweed mitogen-stimulated mouse spleen cells. As little as 0.1% of the conditioned medium is sufficient to promote raspberry colony growth. Under these conditions, nude mouse bone marrow yields as many colonies (1 per 1,000 nucleated cells plated) as normal marrow. Thymus, lymph node; and spleen (normal or nude) do not form colonies. Colony precursors are predominantly in S phase of the cell cycle, as determined by tritiated thymidine suicide of fresh bone marrow. Their numbers fall with age. Because the cells in colonies are Thy-1 positive, peanut agglutinin-positive, and active in a pre-T cell synergy assay, we conclude that their precursors are early committed T cell progenitors, and propose that ...Continue Reading

References

Jun 1, 1979·The Journal of Experimental Medicine·S GillisK A Smith
Jan 1, 1979·Developmental and Comparative Immunology·J LondonM Papiernik
Dec 1, 1979·The Journal of Experimental Medicine·H O BesedovskyE Sorkin
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J J Cohen, S S Fairchild
Oct 1, 1977·Annals of Internal Medicine·D Metcalf
Dec 1, 1975·The Journal of Experimental Medicine·D MetcalfG A Gutman
Aug 1, 1976·The Journal of Experimental Medicine·J J Cohen, S S Fairchild

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