An in vitro coculture model to study cytokine profiles of natural killer cells during maternal immune cell-trophoblast interactions

Journal of the Society for Gynecologic Investigation
Evangelos NtrivalasAlice Gilman-Sachs

Abstract

The cytokine milieu at the implantation site plays a role in human pregnancy. Th2 cytokines, such as interleukin (IL)-4 and IL-10, stimulate growth and development of placenta, whereas Th1 cytokines, such as tumor necrosis factor-alpha (TNF-alpha), are associated with pregnancy complications. Natural killer (NK) cells predominate at the implantation site. The aim of the present study is to investigate cytokine expression in NK cells when they are in close contact with JEG-3 trophoblast-like cells using an in vitro coculture model. Female peripheral blood mononuclear cells (PBMCs) were cocultured with JEG-3 cells for 24 hours. PBMCs were harvested from the cocultures and stimulated with 25 ng/mL phorbol myristate acetate and 1 micromol/mL ionomycin in the presence of 2 micromol/mL monensin. NK cells were analyzed by flow cytometry for intracellular TNF-alpha, interferon-gamma (IFN-gamma), and IL-4 and IL-10 cytokines. Controls were PBMCs cultured without JEG-3 cells. The proportion of CD56+/TNF-alpha(+) NK cells was significantly decreased when they were in coculture with JEG-3 cells (26.1%) as compared to without JEG-3 cell coculture (40.8%) (P < .05). There was no difference in the proportion of CD56(+) NK cells expressing int...Continue Reading

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Citations

Jun 19, 2015·International Journal of Molecular Sciences·Jie XuHui Zhu
Mar 28, 2007·International Immunopharmacology·Jianhong ZhangZhigang Tian
Sep 16, 2009·The Journal of Obstetrics and Gynaecology Research·Joanne Kwak-KimAlice Gilman-Sachs
Jan 10, 2012·American Journal of Reproductive Immunology : AJRI·Mukesh K JaiswalKenneth D Beaman
Sep 22, 2015·Bulletin of Experimental Biology and Medicine·D I SokolovS A Sel'kov
Oct 22, 2013·Veterinary Immunology and Immunopathology·C L SchnabelJ-M V Cavalleri
Aug 8, 2021·Journal of Clinical Medicine·Joana CostaArti Ahluwalia

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