An in vitro evaluation of leukocyte-poor red blood cell concentrates prepared by reconstitution with phosphate buffer solution

The Tohoku Journal of Experimental Medicine
T ShimizuT Fukuda


An effective method for removing leukocytes and platelets from red blood cell concentrates (RBC) reconstituted with phosphate buffer solution was investigated in vitro. Reconstituted RBC were warmed at 37 degrees C for 1 hr. Neither leukocyte count nor platelet count was decreased by the treatment. When compared with whole blood, leukocytes present in the fractions below the buffy coat layer were significantly less than those of whole blood, while the distribution of platelets in reconstituted RBC was unchanged. It is suggested that the reduction of leukocytes in red blood cells below the buffy coat layer may contribute to the preparation of RBC with a poor leukocyte.


Related Concepts

Phosphoric acid, disodium salt, heptahydrate
Cell Separation
Red Blood Cell Count Measurement
Hematocrit Procedure
Hematology (Discipline)
Differential White Blood Cell Count Procedure
Platelet Count Measurement

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