PMID: 8582080Oct 1, 1995Paper

An in vitro growth study on cholesteatoma and normal skin

Clinical Otolaryngology and Allied Sciences
I M CheshireD W Proops

Abstract

The ability of cholesteatoma to grow rapidly within the ear is well recognized. This study aimed to quantify the in vitro growth of cholesteatoma derived cells. Following removal of cholesteatoma explant cultures were established. Cellular outgrowths were subcultured and a colony forming assay performed. Cells were repeatedly passaged every 14 days until senescence was observed. In comparison with cells derived from normal scalp skin, cholesteatoma derived cells demonstrated a lower colony forming efficiency in both primary and secondary subcultures, achieved fewer passages and cell generations in serial culture, and achieved a lower total population expansion. No evidence was found to suggest an intrinsic loss of growth control. It is proposed that the majority of cholesteatoma is composed of cells with a limited capacity for growth. Explant studies suggested that these may be the progeny of more highly proliferative cells situated at the neck of the cholesteatoma sac.

References

Jul 1, 1975·The Journal of Investigative Dermatology·M A Karasek
Sep 1, 1991·American Journal of Otolaryngology·B AbergS Edström
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·Y Barrandon, H Green
Mar 1, 1973·The British Journal of Dermatology·R Marks, T Nishikawa
Jun 1, 1983·Clinical Otolaryngology and Allied Sciences·D W Proops, E K Parkinson
Apr 1, 1981·Clinical Otolaryngology and Allied Sciences·P BretlauP Holmstrup

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Citations

Apr 7, 2012·Histochemistry and Cell Biology·Salvatore RaffaMaurizio Barbara
Nov 22, 2013·Acta Oto-laryngologica·Mette Bendixen ThorupTherese Ovesen
Apr 21, 2001·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·R A CholeD Magilke
Feb 9, 2007·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·Mélanie SanjuanJacques Magnan
Dec 11, 2002·Clinical Otolaryngology and Allied Sciences·M MotamedA R Banerjee
Jan 21, 2010·Acta Oto-laryngologica·Tore HelgalandFlemming S Vassbotn
May 13, 2009·Clinical and Experimental Otorhinolaryngology·Alexander M RaynovKeehyun Park
Apr 20, 2018·Scientific Reports·Julia NagelHolger Sudhoff

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