An in vitro method to study the effects of thyroid hormone-disrupting chemicals on neuronal development

Neurotoxicology
Yu XiongNoriyuki Koibuchi

Abstract

Thyroid hormones (THs) play critical roles for normal cerebellar development. It has been reported that several environmental chemicals may affect cerebellar development through TH system. One such example is the suppression of TH receptor (TR)-mediated transcription by polybrominated diphenyl ethers (PBDEs). To determine the effect of these chemicals on brain development, we established a primary culture system of rat cerebellar Purkinje cells. Using this system, as low as 10(-10)M TH induced Purkinje cell dendritic arborization and such effect was dose-dependent. We examined the effect of decabromodiphenyl ether (BDE209) using this system. Dendritic development of the Purkinje cells was suppressed by 10(-10)M BDE209, that was compatible to the result of the suppression of TR-mediated transcription by using reporter gene assay. These results suggest that TH plays a pivotal role in the development of the Purkinje cell dendrites. Together with in vitro assay system such as reporter gene assay and liquid chemiluminescent DNA-pull down assay, an in vitro protein-DNA binding assay, these assay systems provide us with precise information about environmental chemicals on brain development.

References

Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D J BradleyC Weinberger
Oct 1, 1996·Thyroid : Official Journal of the American Thyroid Association·P J Davis, F B Davis
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·C C Thompson, M C Bottcher
Aug 1, 1997·Endocrine Reviews·J H Oppenheimer, H L Schwartz
May 14, 1999·Current Opinion in Genetics & Development·L XuM G Rosenfeld
Feb 7, 2001·Trends in Endocrinology and Metabolism : TEM·N Koibuchi, W W Chin
Jul 6, 2000·Trends in Endocrinology and Metabolism : TEM·Y Wu, R J Koenig
Nov 26, 2002·Biochemical and Biophysical Research Communications·Toshiharu IwasakiNoriyuki Koibuchi
Feb 18, 2004·The Cerebellum·Noriyuki KoibuchiWilliam W Chin
Jul 11, 2006·Toxicology and Applied Pharmacology·Rocío F CantónMartin van den Berg
Oct 2, 2007·Neurotoxicology·Lucio G Costa, Gennaro Giordano
Nov 21, 2007·Toxicology and Applied Pharmacology·Rocío F CantónMartin van den Berg
Feb 6, 2008·Toxicology Letters·Yuhe HePaul K S Lam
Mar 5, 2008·International Journal of Andrology·P O Darnerud
Jul 10, 2008·The Cerebellum·Noriyuki Koibuchi

❮ Previous
Next ❯

Citations

Sep 28, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Milou M L DingemansMartin van den Berg

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes in Repair & Regeneration

Astrocytes are glial cells found within the CNS and are able to regenerate new neurons. They become activated during CNS injury and disease. The activation leads to the transcription of new genes and the repair and regeneration of neurons. Discover the latest research on astrocytes in repair and regeneration here.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.