PMID: 9655209Jul 9, 1998Paper

An in vitro model for acoustic overstimulation

Acta Oto-laryngologica
A FridbergerM Ulfendahl

Abstract

Although many studies have been performed on the effects of acoustic overstimulation on the inner ear, our knowledge about the cellular processes underlying reduced hearing sensitivity and auditory cell death is still limited. In order to further our understanding of cellular processes occurring in conjunction with acoustic trauma, we designed an in vitro model to study the effects of overstimulation directly on sensory hair cells isolated from the low-frequency part of the guinea pig cochlea. The isolated outer hair cells were subjected to pressure jets delivered by a glass micropipette positioned close to the cell, in order to mimic the pressure changes occurring in the intact inner ear during sound stimulation. A second micropipette coupled to a piezoresistive pressure transducer was used as a probe measuring the pressure at precise locations at and around the cell. In a previous study, we found that such stimulation gave rise to increases in the intracellular calcium concentration. The present study characterizes the stimulus, describes the computer-controlled setup used for calibration, and gives examples of different modes of overstimulation at the cellular level. The peak pressure that could be generated using the pressu...Continue Reading

References

Nov 1, 1992·Hearing Research·G W HardingB A Bohne
Nov 1, 1989·The Journal of the Acoustical Society of America·J C Saunders, Y M Szymko
Jan 1, 1986·Hearing Research·S M Khanna, D G Leonard
Dec 1, 1971·The Annals of Otology, Rhinology, and Laryngology·W D Ward, A J Duvall

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Citations

Apr 13, 2002·Hearing Research·Dalian DingRichard J Salvi
Mar 27, 2007·NeuroImage·Igor TomoMats Ulfendahl
Aug 30, 2018·Physiological Reviews·Jing Wang, Jean-Luc Puel

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