An in vitro model for the assessment of manganese neurotoxicity

Toxicology in Vitro : an International Journal Published in Association with BIBRA
M V VettoriA Mutti

Abstract

PC12 (undifferentiated and differentiated) and C6 cells have been used to investigate kinetics, morphological and functional endpoints following exposure to MnCl(2) and manganic transferrin (Mn-Tf). [Mn](i) in undifferentiated (non-differentiated cells) exposed to both free (MnCl(2)) and bound Mn (Mn-Tf), was three- to fivefold lower as compared to differentiated (differentiated) PC12 cells and higher by one order of magnitude as compared to glial C6 cells. Exposure to both MnCl(2) and Mn-Tf was followed by time- and dose-dependent morphological changes characteristic of apoptosis, which was never observed in Mn-exposed C6 glial cells. Results from cell viability assays were consistent with apoptotic response rates quantified by cell count. Threshold concentrations for undifferentiated and differentiated PC12 cells were 10(-6) and 10(-5)m, respectively. Thus, despite their greater ability to accumulate Mn, differentiated PC12 cells are less sensitive to Mn-induced apoptosis. This model might be relevant to neuronal degeneration induced by Mn occurring in the developing brain and possibly in clinical manganism. Such critical doses at the cellular level seem to be consistent with Mn levels (5x10(-6)m) recorded in the basal gangli...Continue Reading

References

Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L A Greene, A S Tischler
Jan 1, 1992·British Journal of Industrial Medicine·H A RoelsR R Lauwerys
Jan 1, 1991·Neuroscience and Biobehavioral Reviews·M Aschner, J L Aschner
Aug 1, 1987·Archives of Biochemistry and Biophysics·F S Archibald, C Tyree
Aug 1, 1987·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·M C NewlandB Weiss
Apr 1, 1981·Physiological Reviews·L S Hurley
Jul 29, 1995·Lancet·D KriegerH Lichtnecker
Mar 18, 1995·Lancet·G Pomier-LayrarguesR F Butterworth
Aug 1, 1993·Scandinavian Journal of Work, Environment & Health·S E ChiaC S Tian
Feb 1, 1994·Environmental Research·D MerglerL Martin
Jun 1, 1993·British Journal of Industrial Medicine·K NelsonC Angle
Jan 1, 1996·Annual Review of Pharmacology and Toxicology·N A Simonian, J T Coyle
May 1, 1996·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·D Mergler
Dec 7, 1995·European Journal of Pharmacology·P GalvaniA Santagostino
Jul 22, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R GattiG C Gazzola

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Citations

Oct 14, 2003·Toxicology and Applied Pharmacology·Wei ZhengJean-Francois Ghersi-Egea
Jul 26, 2006·Journal of Applied Toxicology : JAT·Fariba SalehiJoseph Zayed
Jul 3, 2003·Risk Analysis : an Official Publication of the Society for Risk Analysis·Matteo GoldoniAntonio Mutti
Nov 3, 2016·Biological Trace Element Research·Saylav BoraTaner Dagcı
Oct 18, 2011·Environmental Research·S MontesC Rios
Jan 11, 2014·The Journal of Physical Chemistry. B·Shiyan XiaoMercedes Alfonso-Prieto
Jan 26, 2011·Brain Research·A P Marreilha Dos SantosM Aschner
Dec 27, 2005·The Science of the Total Environment·Chadi YazbeckGuy Huel
Feb 24, 2012·The Journal of Biological Chemistry·Yuanyuan ChenPhilip D Kiser
Jul 20, 2017·The Journal of Endocrinology·William L DeesVinod K Srivastava
Sep 14, 2007·Alternatives to Laboratory Animals : ATLA·Enrico BignettiGaetano Aiello

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