An in vitro model of the urinary bladder: cultured porcine urinary bladder epithelial cells

Toxicology in Vitro : an International Journal Published in Association with BIBRA
W FöllmannC Guhe

Abstract

Epithelial cells from urinary bladders of pigs were isolated and cultured under serum-free conditions. For these cells it was previously shown that they developed morphologic polarity resembling the epithelium in vivo. Lactate dehydrogenase release was low, chromosome set and activities of marker enzymes (alkaline phosphatase, acid phosphatase, g-glutamyltranspeptidase) were stable over a period of 4 wk. In this study, metabolic competence was evaluated by measuring activities of phase I and phase II enzymes. Activity of prostaglandin H-synthase was expressed in freshly isolated cells as well as in cultured cells, as were activities of the conjugating enzymes glutathione transferase, UDP-glucuronyltransferase and N-acetyltransferase. Cytochrome P4501A1 activity in freshly isolated cells amounted to 10-15% of the respective activity in the porcine liver, this activity was not detectable in cultured cells. No activity was seen in cultured cells after induction with methylcholanthrene and benz[a]anthracene. This cell culture system was used to detect genotoxic effects of substances suspected to induce bladder cancer by measuring the induction of sister chromatid exchanges (SCE). The aromatic amines 4-aminobiphenyl and 2-aminofluor...Continue Reading

References

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Citations

Nov 14, 2002·Urology·Guido DalbagniVictor E Reuter
Dec 25, 2003·Urologic Oncology·H Barton Grossman, Colin P N Dinney
Oct 12, 2005·The Journal of Urology·Charalampos AndreadisDespina Mouratidou
Dec 17, 2008·The Journal of Urology·Chung-Hsin ChenYeong-Shiau Pu

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