An In Vitro Oxygen-Glucose Deprivation Model for Studying Ischemia-Reperfusion Injury of Neuronal Cells

Methods in Molecular Biology
Myoung-Gwi Ryou, Robert T Mallet

Abstract

Ischemia-reperfusion syndromes of the heart and brain are the leading cause of death and long-term disability worldwide. Development of effective treatments for myocardial infarction, stroke, cardiac arrest and their sequelae requires preclinical models that replicate specific features of ischemia-reperfusion. The complexities of intact animals, including the integrated function of organ systems, autonomic innervation and endocrine factors, often preclude detailed study of specific components of ischemia-reperfusion injury cascades. Ischemia represents the interruption of metabolic fuel and oxygen delivery to support cellular oxidative metabolism; reintroduction of oxygen upon reperfusion of ischemic tissue triggers oxidative stress which initiates the reperfusion injury cascade culminating in injury and death of cells and tissues. Thus, cultured cells subjected to hypoxia, fuel deprivation and reoxygenation replicate the cardinal features of ischemia-reperfusion, while accommodating interventions such as siRNA suppression of specific genes and pharmacological activation or inhibition of signaling cascades that are not feasible in more complex preparations, especially intact animals. This chapter describes an in vitro OGD-reoxy...Continue Reading

Citations

Mar 1, 2019·International Journal of Molecular Sciences·Valeria GasperiMaria Valeria Catani
Oct 8, 2020·Stem Cells Translational Medicine·Vanessa CastelliAnnamaria Cimini
Oct 28, 2020·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Huijun Wen, Maikou Lv
Mar 5, 2021·Human & Experimental Toxicology·P LiX Huang
Mar 5, 2021·Reviews in the Neurosciences·Harish IyerArvind Kumar
Aug 8, 2021·International Journal of Molecular Sciences·Bhakta Prasad Gaire, Ji-Woong Choi

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