An in vitro procedure for studying enzymic dephosphorylation of phytate in maize-soyabean feeds for turkey poults

The British Journal of Nutrition
Krzysztof ZyłaT L Veum

Abstract

An in vitro method was developed to predict inorganic P release from maize-soyabean poultry feeds containing supplemental phytase (EC 3.1.3.8), and to quantify the effect of acid phosphatase (EC 3.1.3.2), fungal protease (EC 3.4.23.6) and Aspergillus niger cellulase (EC 3.2.1.4) on phytate dephosphorylation. Pepsin (EC 3.4.23.1) and pancreatin digestion periods were preceded by a 30 min pre-incubation at pH 5.25 to simulate digestion in the crop of poultry. Pancreatin digestion was carried out in dialysis tubing, with a ratio of about 1:25 (v/v) between the digesta and dialysing medium, to simulate gradient absorption from the duodenum. The feed:water ratio was kept within physiological limits and a constant proportion of feed weight to digestive enzymes was maintained. There was a linear response to increasing dosages of phytase up to 1000 phytase units (FTU)/kg feed, and to increasing phosphate concentration in feeds. In vivo validation was performed with growing turkeys (1-3 weeks) fed on diets containing 12 g Ca/kg and 0, 500 or 1000 FTU phytase/kg in a factorial arrangement with 0, 1, 2 or 3 g supplemental phosphate/kg (from KH2PO4). After a simple transformation (variable/in vitro P = f (in vitro P)), amounts of P hydroly...Continue Reading

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Citations

Nov 20, 1998·Folia Microbiologica·J Dvoráková
Mar 2, 2017·Journal of the Science of Food and Agriculture·Vera SommerfeldMarkus Rodehutscord
Jul 27, 2007·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Debbie Y ChanBeverley A Hale
Dec 19, 2020·Journal of the Science of Food and Agriculture·Vera Sommerfeld, Regiane R Santos
Apr 9, 2021·Biochemistry. Biokhimii︠a︡·Valerij S KryukovSergej V Zinoviev
May 1, 2021·Animals : an Open Access Journal From MDPI·Gerald C ShursonPedro E Urriola
Sep 21, 2019·Journal of Agricultural and Food Chemistry·Johanna HirvonenPäivi Nurminen
Sep 20, 2000·Journal of Agricultural and Food Chemistry·A ViverosA Lozano

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