An in vitro proof-of-principle study of sonobactericide

Scientific Reports
Kirby R LattweinKlazina Kooiman

Abstract

Infective endocarditis (IE) is associated with high morbidity and mortality rates. The predominant bacteria causing IE is Staphylococcus aureus (S. aureus), which can bind to existing thrombi on heart valves and generate vegetations (biofilms). In this in vitro flow study, we evaluated sonobactericide as a novel strategy to treat IE, using ultrasound and an ultrasound contrast agent with or without other therapeutics. We developed a model of IE biofilm using human whole-blood clots infected with patient-derived S. aureus (infected clots). Histology and live-cell imaging revealed a biofilm layer of fibrin-embedded living Staphylococci around a dense erythrocyte core. Infected clots were treated under flow for 30 minutes and degradation was assessed by time-lapse microscopy imaging. Treatments consisted of either continuous plasma flow alone or with different combinations of therapeutics: oxacillin (antibiotic), recombinant tissue plasminogen activator (rt-PA; thrombolytic), intermittent continuous-wave low-frequency ultrasound (120-kHz, 0.44 MPa peak-to-peak pressure), and an ultrasound contrast agent (Definity). Infected clots exposed to the combination of oxacillin, rt-PA, ultrasound, and Definity achieved 99.3 ± 1.7% loss, wh...Continue Reading

References

Nov 1, 1979·Progress in Cardiovascular Diseases·L R Freedman, J Valone
Jul 1, 1989·DICP : the Annals of Pharmacotherapy·R T Scheife
Dec 1, 1985·Proceedings of the Society for Experimental Biology and Medicine·W M ScheldR A Calderone
Jan 1, 1969·Cardiovascular Research·S Rodbard, C Yamamoto
Sep 8, 1998·Journal of Biomedical Materials Research·Y H An, R J Friedman
Oct 19, 1999·Journal of Clinical Microbiology·B ShopsinB N Kreiswirth
Sep 5, 2001·Progress in Cardiovascular Diseases·E C UngerT McCreery
Jan 1, 1963·Circulation·S RODBARD
Nov 15, 2003·The Journal of Pediatrics·Aviva LevitasKyla A Marks
Mar 12, 2004·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·P S McKinnon, S L Davis
Oct 14, 2004·The Journal of the Acoustical Society of America·Damir B Khismatullin
Mar 12, 2005·American Journal of Infection Control·John C CarmenWilliam G Pitt
Apr 6, 2005·Biochemistry. Biokhimii︠a︡·K Lewis
May 23, 2006·Pediatric Blood & Cancer·Adalet Meral GunesEvren Semizel
Aug 1, 2006·Ultrasound in Medicine & Biology·Saurabh DattaChristy K Holland
Sep 19, 2006·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Gaetano Thiene, Cristina Basso
Oct 17, 2006·Journal of Bacteriology·Victor J TorresEric P Skaar
Mar 6, 2007·Ultrasound in Medicine & Biology·Jason M MeunierGeorge J Shaw
Mar 29, 2007·Journal of Thrombosis and Haemostasis : JTH·N J MutchN A Booth
Dec 15, 2007·Acoustics Research Letters Online : ARLO·Jason Y ChengChristy K Holland
May 28, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Rachel J Gordon, Franklin D Lowy
May 13, 2008·Ultrasound in Medicine & Biology·George J ShawChristy K Holland
Apr 21, 2009·Applied and Environmental Microbiology·Akinobu ItoSatoshi Okabe
Sep 17, 2011·Virulence·Nathan K ArcherMark E Shirtliff
Nov 30, 2011·Microbes and Infection·Kathryn P Haley, Eric P Skaar
May 23, 2012·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Franci Bajd, Igor Serša
Nov 17, 2012·Journal of Microbiological Methods·Jonathan Hon-Kwan ChenKwok-Yung Yuen
Dec 19, 2012·The Journal of Antimicrobial Chemotherapy·Ying DongJialin Yu
Feb 21, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Akhil K SethRobert D Galiano
Mar 2, 2013·Expert Opinion on Drug Delivery·Jonathan T SuttonChristy K Holland
Mar 12, 2013·Biophysical Journal·Franci Bajd, Igor Serša
Apr 12, 2013·The New England Journal of Medicine·Bruno Hoen, Xavier Duval

❮ Previous
Next ❯

Citations

Jun 13, 2020·Scientific Reports·Chikahiro ImashiroKenjiro Takemura
Nov 9, 2019·Ultrasound in Medicine & Biology·Kirby R LattweinKlazina Kooiman
Mar 14, 2020·Ultrasound in Medicine & Biology·Klazina KooimanChristy K Holland
Jul 24, 2021·Cellular and Molecular Life Sciences : CMLS·Yi-Ju HoChih-Kuang Yeh
Jan 19, 2021·ACS Biomaterials Science & Engineering·Rajeet ChandanRinti Banerjee
Oct 30, 2021·ACS Biomaterials Science & Engineering·Jayishnu RoyHimanshu Shekhar

❮ Previous
Next ❯

Methods Mentioned

BETA
light microscopy
confocal microscopy
biopsy

Software Mentioned

NIS
ImageJ
MATLAB
Elements
GraphPad Prism

Related Concepts

Related Feeds

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.