Abstract
To investigate the precipitation process of a mixture of vancomycin, amikacin, and dexamethasone by equilibrium dialysis and its subsequent effect on the levels of available-free antibiotics and steroid. Concentrations of amikacin, vancomycin, and dexamethasone in an equilibrium dialysis chamber were measured during the equilibrium process by high-performance liquid chromatography and fluorescence polarisation immunoassay. Vitreous were used as the medium of dialysis, with the three medications prepared in normal saline (NS) and balanced salt solution plus (BSS Plus) separately. Amikacin showed no measurable loss in NS or BSS Plus, either alone or when mixed with vancomycin or dexamethasone. Vancomycin showed minimal loss in BSS Plus, either alone or when mixed with amikacin or dexamethasone. Dexamethasone showed a median loss of 16 and 15% when incubated alone in NS and BSS Plus, respectively, at 48 h. When mixed with vancomycin or amikacin in BSS Plus, it showed a median loss of 13 and 12%, respectively, at 48 h. There was no statistically significant difference in the loss of dexamethasone under various conditions. In equilibrium dialysis in vitreous, amikacin, vancomycin, and dexamethasone reached equilibrium within 24 h an...Continue Reading
References
Jul 1, 1991·Antimicrobial Agents and Chemotherapy·M A SmithM Borenstein
Jul 1, 1990·The Journal of Infectious Diseases·S K ShraderP Murphy
Sep 1, 1984·Journal of Clinical Microbiology·M A PfallerP R Murray
Nov 1, 1984·Journal of Clinical Microbiology·J H Ngui-YenJ A Smith
Apr 1, 1984·Archives of Ophthalmology·M S BlumenkranzA S Hajek
Oct 1, 1995·Archives of Ophthalmology·S S ParkA S Baker
Jan 1, 1994·Archives of Ophthalmology·S P DonahueB H Jewart
Jan 1, 1994·Archives of Ophthalmology·P A Campochiaro, J I Lim
Jun 1, 1993·Archives of Ophthalmology·R G Fiscella
Jun 1, 1996·Clinics in Chest Medicine·D PayenS Beloucif
Dec 1, 1995·Korean Journal of Ophthalmology : KJO·H W Kwak, D J D'Amico
Mar 1, 1996·The Journal of Applied Bacteriology·N Cochet, A L Demain
Aug 17, 1999·Archives of Ophthalmology·S S ParkD J D'Amico
Aug 24, 1999·American Journal of Ophthalmology·D Y KunimotoT N Rao
Aug 24, 1999·American Journal of Ophthalmology·D Y KunimotoT N Rao
Aug 26, 1999·The British Journal of Ophthalmology·T DasT J Naduvilath
Nov 5, 1999·Retina·M O YoshizumiA Kashani
Feb 24, 2000·The British Journal of Ophthalmology·Y AlsterA Loewenstein
Feb 26, 2000·The British Journal of Ophthalmology·T LifshitzI Klemperer
Jan 1, 2004·American Journal of Ophthalmology·Matthew S BenzDarlene Miller
Jan 23, 2004·The British Journal of Ophthalmology·M HuiA F B Cheng
Jul 10, 2004·Trends in Molecular Medicine·Dominique HeymannFrançoise Rédini