PMID: 9549623Apr 29, 1998Paper

An in vitro system for studying osteointegration of dental implants utilizing cells grown on dense hydroxyapatite disks

Journal of Biomedical Materials Research
D L Layman, R C Ardoin

Abstract

A proposed in vitro system is described where chick osteoblasts are cultured on the flat surfaces of dense, nonporous HA disks to facilitate the study of bone formation at the cell-HA interface. During early bone formation cell-coated HA disks were retrieved, fixed with buffered 2% glutaraldehyde, and embedded in epon/araldite. The underlying HA disks were demineralized in diluted acid, and the intact cell-HA interfaces were re-embedded and thin sectioned for routine transmission electron microscopy. Morphologic studies indicated that osteoblasts proliferated and formed nodules of cells on the surfaces of HA disks. With increasing time in culture, they deposited orthogonally packed collagen fibrils between the cell layers that were enveloped by electron-dense mineralized globules. Eventually, small spicules of mineralized HA formed along collagen fibrils. An electron-dense layer about 50 nm thick was observed on the surface of the HA disks. Biochemical studies indicated that cell proliferation, as judged by 3H-thymidine uptake, increased rapidly during the first 3 days, reached a maximum around 6 days, and then declined by 12 days in culture. AP activity and collagen synthesis, as determined by 3H-hydroxyproline formation, incr...Continue Reading

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Citations

Nov 15, 2000·Journal of Biomedical Materials Research·S SofiaD L Kaplan
Jan 17, 2002·Biomaterials·Sang-Hoon Rhee
Oct 20, 2005·Journal of Bioscience and Bioengineering·T SuzukiM Toriyama
Jan 19, 2005·Cells, Tissues, Organs·Stefan RammeltHans Zwipp
Mar 20, 2013·Biomatter·Yvonne FörsterStefan Rammelt
Aug 25, 2011·Journal of Biomaterials Applications·Kien-Seng LewFei-Yee Yeoh
Mar 30, 2001·Advances in Dental Research·D M Brunette
Aug 11, 2005·Journal of Biomedical Materials Research. Part a·S Sh HomaeigoharM Hosseinalipour
Jul 10, 2007·Journal of Biomedical Materials Research. Part a·S Sh HomaeigoharA Yari Sadi
Jan 26, 2005·Journal of Biomedical Materials Research. Part a·Tony ParkerDavid Leavesley

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