An in vitro test of the cell stretch-proliferation hypothesis of renal cyst enlargement.

Journal of the American Society of Nephrology : JASN
G A TannerJ A McAteer

Abstract

In renal cystic disease, fluid accumulation within cyst lumens might stretch cyst walls and in this way stimulate cell proliferation. To test this idea, the effects of mechanical stretch on Madin-Darby canine kidney cells grown as cysts in a hydrated collagen gel or as monolayers on collagen-coated Flexcell membranes were examined. The percentage of cells synthesizing DNA (labeling index) was determined by measuring bromodeoxyuridine incorporation and counting cell numbers. The distension of single cysts for 1 h by the intraluminal injection of saline failed to produce a significant increase in labeling index. The exposure of cysts for 2.5 h to 1 mM dibutyryl cAMP + 0.1 mM isobutylmethylxanthine led to a 37% increase in luminal surface area (due to stimulated fluid secretion) and a 30% increase in labeling index. The stretch (25%) of Madin-Darby canine kidney monolayers approximately doubled the labeling index between 12 and 24 h after starting the stretch. After 48 h, the cell population density was significantly increased (P < 0.001), from 41.9 +/- 0.2 (SE; N = 12) to 48.2 +/- 0.5 (N = 12) cells/10,000 microns2. The labeling index increased linearly with applied stretch, from 7.2 +/- 0.3% (N = 36) with no stretch to 16.2 +/- ...Continue Reading

Citations

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Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Lars HufnagelBoris I Shraiman
Sep 1, 1996·Kidney International·G A TannerM Steinhausen
Jan 18, 2008·American Journal of Physiology. Cell Physiology·Claudia R AmuraR Brian Doctor
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Oct 13, 2010·Proceedings of the National Academy of Sciences of the United States of America·Nathan E HellmanIain A Drummond
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Mar 12, 2011·Biochimica Et Biophysica Acta·Xiaohong Li

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