PMID: 9177208Jun 10, 1997Paper

An in vitro tubulogenesis system using cell lines derived from the embryonic kidney shows dependence on multiple soluble growth factors

Proceedings of the National Academy of Sciences of the United States of America
Hiroyuki SakuraiS Nigam

Abstract

Interactions between the ureteric bud (UB) and metanephric mesenchyme are crucial for tubulogenesis during kidney development. Two immortalized cell lines derived from the day 11.5 embryonic kidney, UB cells, which appear to be epithelial (cytokeratin-positive, E-cadherin-positive, and ZO-1-positive by immunostaining) and BSN cells, which are largely mesenchymal (vimentin-positive, but negative for cytokeratin, cell surface E-cadherin, and cell surface ZO-1), were used to establish an in vitro tubulogenesis system. BSN cells expressed hepatocyte growth factor (HGF) and transforming growth factor-beta1 mRNAs, and its conditioned medium (BSN-CM) contained factors capable of activating the epidermal growth factor (EGF) receptor (EGFR). When UB cells were cultured in an extracellular matrix gel in the presence of the embryonic kidney or BSN-CM, the UB cells underwent morphogenetic changes characteristic of early in vitro branching tubulogenesis. These changes were largely inhibited by a combination of neutralizing anti-HGF antibodies and the EGFR inhibitor tyrphostin AG1478, suggesting that EGFR ligands, together with HGF, account for much of this early morphogenetic activity. Nevertheless, there was a significant fraction of tubul...Continue Reading

References

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Citations

Oct 30, 2008·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Ihor V Yosypiv
Dec 18, 2007·Journal of Molecular Histology·Dominko CarevMirna Saraga-Babic
Jun 18, 2003·Biochemical and Biophysical Research Communications·Zhixing LiSanjay K Nigam
Apr 2, 2003·Trends in Cell Biology·Mirjam M P ZegersKeith E Mostov
Nov 25, 2003·Seminars in Cell & Developmental Biology·Hiroyuki Sakurai
Dec 16, 1998·Kidney International·Q al-Awqati, M R Goldberg
Apr 23, 2002·Kidney International·Frank StrutzEric G Neilson
May 20, 2003·Kidney International·Zoia B LevashovaAlan O Perantoni
Apr 5, 2003·Kidney International·Katherine K RogersJoshua H Lipschutz
Mar 8, 2007·Tissue Engineering·Cindy WeiKenneth M Yamada
Feb 10, 2006·Molecular Biology of the Cell·Ambra PozziRoy Zent
Aug 9, 2003·Critical Care Medicine·Kathleen D Liu
Jun 9, 2000·Annual Review of Physiology·M PohlS K Nigam
Mar 25, 2011·American Journal of Physiology. Renal Physiology·Zhiguo MaoAlbert C M Ong
May 15, 2009·American Journal of Physiology. Renal Physiology·Wei WuSanjay K Nigam
Jun 4, 2011·Journal of Signal Transduction·Renfang SongIhor V Yosypiv
Nov 19, 2009·Cancer Research·Christopher JedeszkoBonnie F Sloane
May 27, 2003·Journal of Cell Science·Karen L Schmeichel, Mina J Bissell
Feb 4, 2014·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Sanjay K Nigam, Kevin T Bush
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·Z LiS K Nigam
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Zhendong MaPeter Igarashi
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·R O StuartS K Nigam
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·J QiaoS K Nigam
Dec 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·E RosinesS K Nigam
Feb 27, 2010·Journal of Cellular and Molecular Medicine·Naomi Pode-ShakkedBenjamin Dekel
Nov 5, 2011·Journal of Tissue Engineering and Regenerative Medicine·Shuang Hong LüChang Yong Wang
Jul 1, 2010·Journal of Cellular and Molecular Medicine·Huishi ZhangDaniele Zink
Jan 15, 2014·The EMBO Journal·Inmaculada Bañón-RodríguezFernando Martín-Belmonte
Jan 31, 2014·Journal of Tissue Engineering and Regenerative Medicine·Peter V HauserNorimoto Yanagawa
Sep 11, 2014·BMC Developmental Biology·Jamie A DaviesRachel Berry

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